Western Blot (WB)
(Histone macroH2A1.1 antibody (pAb) tested by Western blot. U2OS cells mock transfected (lane 1) or transfected with tagged macroH2A1.1 (lane 2), macroH2A1.2 (lane 3) or macroH2A2 (lane 4) were probed with Histone macroH2A1.1 antibody at a dilution of 1:1,000.)
Rabbit anti-Human Histone macroH2A1.1 Polyclonal Antibody | anti-macroH2A1.1 antibody
Histone macroH2A1.1 antibody (pAb)
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
Histone macroH2A1.1; Polyclonal Antibody; Histone macroH2A1.1 antibody (pAb); anti-macroH2A1.1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-macroH2A1.1 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:1,000 dilution
WB: 1:500-1:1,000 dilution
Immunogen
This macroH2A1.1 antibody was raised against a peptide corresponding to amino acids 198-226 of human macroH2A1.1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Histone macroH2A1.1 antibody (pAb) tested by Western blot. U2OS cells mock transfected (lane 1) or transfected with tagged macroH2A1.1 (lane 2), macroH2A1.2 (lane 3) or macroH2A2 (lane 4) were probed with Histone macroH2A1.1 antibody at a dilution of 1:1,000.)
Western Blot (WB)
(Histone macroH2A1.1 antibody (pAb) tested by Western blot. U2OS cells mock transfected (lane 1) or transfected with tagged macroH2A1.1 (lane 2), macroH2A1.2 (lane 3) or macroH2A2 (lane 4) were probed with Histone macroH2A1.1 antibody at a dilution of 1:1,000.)
Related Product Information for anti-macroH2A1.1 antibody
Short Description: Our Histone macroH2A1.1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western blot; it has been shown to react with Human samples.
Background: Histone macroH2A1.1 is a histone H2A variant and one of the three members of the macroH2A family. The three macroH2A proteins contain the C-terminal 'macro' domain in addition to the H2A homologous region. This macro domain may bind poly ADP-ribose and other NAD+ metabolites. The macroH2A1.1 and macroH2A1.2 proteins are produced from alternatively spliced transcripts from the macroH2A1 gene while a second distinct macroH2A gene encodes macroH2A2. In mammals, macroH2A is preferentially localized to the inactive X chromosome and in general is proposed to play a role in repressing gene expression. In mice, macroH2A1 is required for the silencing of endogenous retroviruses.
Background: Histone macroH2A1.1 is a histone H2A variant and one of the three members of the macroH2A family. The three macroH2A proteins contain the C-terminal 'macro' domain in addition to the H2A homologous region. This macro domain may bind poly ADP-ribose and other NAD+ metabolites. The macroH2A1.1 and macroH2A1.2 proteins are produced from alternatively spliced transcripts from the macroH2A1 gene while a second distinct macroH2A gene encodes macroH2A2. In mammals, macroH2A is preferentially localized to the inactive X chromosome and in general is proposed to play a role in repressing gene expression. In mice, macroH2A1 is required for the silencing of endogenous retroviruses.
Product Categories/Family for anti-macroH2A1.1 antibody
NCBI and Uniprot Product Information
Molecular Weight
41kDa
Similar Products
Product Notes
The macroH2A1.1 (Catalog #AAA388614) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone macroH2A1.1 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone macroH2A1.1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution. Researchers should empirically determine the suitability of the macroH2A1.1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone macroH2A1.1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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