Testing Data #2
(Fig2: Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7 or 8. Recognition of CEACAM3 was tested on stably transfected HeLa cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3 (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified 9A6 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM6-expressing cells (red curves).)
Mouse anti-Human CEACAM6 Monoclonal Antibody | anti-CEACAM6 antibody
anti-human CEACAM6 monoclonal antibody
ELISA: 1:200 - 1:400
Western Blot: 4ug/ml
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400
Testing Data #2
(Fig2: Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7 or 8. Recognition of CEACAM3 was tested on stably transfected HeLa cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3 (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified 9A6 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM6-expressing cells (red curves).)
Testing Data #2
(Fig2: Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7 or 8. Recognition of CEACAM3 was tested on stably transfected HeLa cells (CEACAM4). Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3 (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified 9A6 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM6-expressing cells (red curves).)
Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin Cancer Biol. 9, 67-81.
Scholzel S, Zimmermann W, Schwarzkopf G, Grunert F, Rogaczewski B and Thompson J (2000). Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas. Am J Pathol 156, 595-605.
Grunert F, Stocks SC, Nagel G., Zimmermann W, Thompson JA, Jantscheff P and Kromer B. (1996). CD66 family Workshop: Binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules. In Leukocyte Typing VI: White cell Differentiation Antigens (T. Kishimoto et al., eds.), Garland Publishing Inc., New York and London, pp. 1012-1025.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that belongs to the carcinoembryonic antigen (CEA) family whose members are glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins. Members of this family play a role in cell adhesion and are widely used as tumor markers in serum immunoassay determinations of carcinoma. This gene affects the sensitivity of tumor cells to adenovirus infection. The protein encoded by this gene acts as a receptor for adherent-invasive E. coli adhesion to the surface of ileal epithelial cells in patients with Crohn's disease. This gene is clustered with genes and pseudogenes of the cell adhesion molecules subgroup of the CEA family on chromosome 19. [provided by RefSeq, Apr 2014]
Uniprot Description
CEACAM6: Belongs to the immunoglobulin superfamily. CEA family
Protein type: Membrane protein, integral; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein binding
Biological Process: cell-cell signaling; blood coagulation; signal transduction; leukocyte migration
Research Articles on CEACAM6
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Product Notes
The CEACAM6 ceacam6 (Catalog #AAA468045) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CEACAM6 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM6 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry, CELISA, ELISA (EIA), Western Blot (WB) and Immunohistology on cryosections. Immunohistology: 1-2 ug/10^6 cells (on cryosections) ELISA: 1:200 - 1:400 Western Blot: 4ug/ml Flow Cytometry: 1.2 ug/10^6 cells CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the CEACAM6 ceacam6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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