Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CDC45 antibody. Counter stained with hematoxylin.)
Rabbit CDC45 Monoclonal Antibody | anti-CDC45 antibody
CDC45 Antibody
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CDC45 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CDC45 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDC45 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDC45 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CDC45 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CDC45 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining CDC45 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining CDC45 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining CDC45 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining CDC45 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining CDC45 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining CDC45 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293 cells with CDC45 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293 cells with CDC45 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
CDC45L: Required for initiation of chromosomal DNA replication. Associated with ORC2. Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. Belongs to the CDC45 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus
Molecular Function: protein binding; 3'-5' DNA helicase activity; DNA replication origin binding; chromatin binding; single-stranded DNA binding
Biological Process: G1/S-specific transcription in mitotic cell cycle; DNA replication initiation; regulation of chromatin silencing at telomere; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication checkpoint; DNA replication; DNA duplex unwinding; double-strand break repair via break-induced replication; G1/S transition of mitotic cell cycle; pre-replicative complex assembly
Research Articles on CDC45
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Product Notes
The CDC45 cdc45 (Catalog #AAA9436031) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC45 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDC45 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the CDC45 cdc45 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC45, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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