Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysateAll lanes: Cdc25C antibody at 1.65ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 54, 52, 49, 46 KDaObserved band size: 60 KDa)
CDC25C recombinant antibody
CDC25C Antibody
IHC: 1:50-1:200
IF: 1:20-1:200
IP: 1:200-1:1000
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysateAll lanes: Cdc25C antibody at 1.65ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 54, 52, 49, 46 KDaObserved band size: 60 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysateAll lanes: Cdc25C antibody at 1.65ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 54, 52, 49, 46 KDaObserved band size: 60 KDa)
Immunohistochemistry (IHC)
(IHC image of CSB-RA004996A0HU diluted at 1:165 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of CSB-RA004996A0HU diluted at 1:165 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with CSB-RA004996A0HU at 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with CSB-RA004996A0HU at 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunoprecipitation (IP)
(Immunoprecipitating CDC25C in HEK293 whole cell lysateLane 1: Rabbit control IgG instead of CSB-RA004996A0HU in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA004996A0HU (3ug) + HEK293 whole cell lysate (500ug)Lane 3: HEK293 whole cell lysate (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating CDC25C in HEK293 whole cell lysateLane 1: Rabbit control IgG instead of CSB-RA004996A0HU in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA004996A0HU (3ug) + HEK293 whole cell lysate (500ug)Lane 3: HEK293 whole cell lysate (20ug))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]
Uniprot Description
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Research Articles on CDC25C
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Product Notes
The CDC25C cdc25c (Catalog #AAA7114797) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC25C Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDC25C can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:200 IP: 1:200-1:1000. Researchers should empirically determine the suitability of the CDC25C cdc25c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC25C, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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