Western Blot (WB)
(All lanes: Glutamine--tRNA ligase antibody at 2ug/mlLane 1:PC-3 whole cell lysateLane 2:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 88,87 kDaObserved band size: 88 kDa)
Rabbit anti-Human Glutamine-tRNA ligase Polyclonal Antibody | anti-QARS antibody
Rabbit anti-human Glutamine-tRNA ligase polyclonal Antibody(QARS)
IHC:1:20-1:200
Western Blot (WB)
(All lanes: Glutamine--tRNA ligase antibody at 2ug/mlLane 1:PC-3 whole cell lysateLane 2:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 88,87 kDaObserved band size: 88 kDa)
Western Blot (WB)
(All lanes: Glutamine--tRNA ligase antibody at 2ug/mlLane 1:PC-3 whole cell lysateLane 2:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 88,87 kDaObserved band size: 88 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human rectum using MBS1492162 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human rectum using MBS1492162 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human rectal cancer using MBS1492162 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human rectal cancer using MBS1492162 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon cancer using MBS1492162 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon cancer using MBS1492162 at dilution 1:100)
Immunoprecipitation (IP)
(Immunoprecipitating QARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1492162 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1492162(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating QARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1492162 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1492162(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
NCBI and Uniprot Product Information
NCBI Description
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
Uniprot Description
QARS: catalyzes the formation of L-glutaminyl-tRNA. Belongs to the class-I aminoacyl-tRNA synthetase family.
Protein type: Mitochondrial; Ligase; EC 6.1.1.18; Translation
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: cytoplasm; cytosol; mitochondrial matrix
Molecular Function: ATP binding; glutamine-tRNA ligase activity; protein binding
Biological Process: brain development; gene expression; glutaminyl-tRNA aminoacylation; selenium metabolic process; tRNA aminoacylation for protein translation
Disease: Microcephaly, Progressive, With Seizures And Cerebral And Cerebellar Atrophy
Research Articles on QARS
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Product Notes
The QARS qars (Catalog #AAA1492162) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Glutamine-tRNA ligase polyclonal Antibody(QARS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamine-tRNA ligase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the QARS qars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamine-tRNA ligase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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