Immunofluorescence (IF)
(Immunofluorescence staining of Human CD9 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD9 monoclonal antibody (1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.)
Mouse anti-Human CD9 Monoclonal Antibody | anti-CD9 antibody
Anti-CD9 Antibody, Mouse Monoclonal
FC/FACS/FCM: 1:25-1:100
Immunofluorescence (IF)
(Immunofluorescence staining of Human CD9 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD9 monoclonal antibody (1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.)
Immunofluorescence (IF)
(Immunofluorescence staining of Human CD9 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD9 monoclonal antibody (1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD9 expression on HeLa cells. Cells were stained with purified anti-Human CD9, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD9 expression on HeLa cells. Cells were stained with purified anti-Human CD9, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
NCBI and Uniprot Product Information
Uniprot Description
CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.
Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.3
Cellular Component: extracellular space; platelet alpha granule membrane; focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane; vesicle; external side of plasma membrane
Molecular Function: integrin binding; protein binding
Biological Process: negative regulation of cell proliferation; platelet activation; platelet degranulation; fusion of sperm to egg plasma membrane; single fertilization; pathogenesis; brain development; cell adhesion; cell motility; blood coagulation; oligodendrocyte development; response to water deprivation; multicellular organism reproduction; paranodal junction assembly
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Product Notes
The CD9 cd9 (Catalog #AAA8104646) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD9 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD9 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:50-1:1000 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the CD9 cd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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