p73 beta recombinant protein

p73 beta recombinant protein

Cat. Num. AAA515244

• Gene Names: TP73; P73
• Applications: Western Blot
• Synonyms: p73 beta; p73 beta recombinant protein; TP73; TAp73alpha; P73b Protein

• Host: Sf9 insect cells
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 587

• Applicable Applications for p73 beta recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: Sf9 insect cells using baculovirus
• Source Note: Full-length recombinant human p73beta was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for p73 beta recombinant protein

Full-length recombinant human p73 beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: p73β is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. P73β is strongly involved in malignancy acquisition and maintenance (1). p73 is a stress-response gene that activates transcription of p53-responsive genes and inhibits cell growth in a p53-like manner by inducing apoptosis (2). p73 is a component of a mismatch repair-dependent apoptosis pathway, which contributes to cisplatin-induced cytotoxicity. The regulation of p73 by c-Abl in response to DNA damage was also demonstrated by a failure of ionizing radiation-induced apoptosis after disruption of c-Abl-p73 interaction.

Product Categories/Family for p73 beta recombinant protein

Cell Cycle Proteins; Signaling Proteins - Cell Cycle Proteins

References

1. Jost. C. A. et.al: p73 is a human p53-related protein that can induce apoptosis. Nature 389: 191-194, 1997.
2. Gong, J. et.al: The tyrosine kinase c-Abl regulates p73 in apoptotic response to cisplatin-induced DNA damage. Nature 399: 806-809, 1999.

NCBI and Uniprot Product Information

• NCBI GI #: 187828902
• NCBI GeneID: 7161
• NCBI Accession #: NP_001119712.1
• NCBI GenBank Nucleotide #: NM_001126240.2
• UniProt Accession #: O15350; O15351; Q17RN8; Q5TBV5; Q5TBV6; Q8NHW9; Q8TDY5; Q8TDY6; Q9NTK8; B7Z7J4; B7Z9C1
• Molecular Weight: ~88 kDa
• NCBI Official Full Name: tumor protein p73 isoform b
• NCBI Official Synonym Full Names: tumor protein p73
• NCBI Official Symbol: TP73
• NCBI Official Synonym Symbols: P73
• NCBI Protein Information: tumor protein p73; p53-related protein; p53-like transcription factor
• UniProt Protein Name: Tumor protein p73
• UniProt Gene Name: TP73
• UniProt Synonym Gene Names: P73
• UniProt Entry Name: P73_HUMAN

NCBI Description

This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined. [provided by RefSeq, Feb 2011]

Uniprot Description

Function: Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. Ref.6 Ref.14 Ref.23

Cofactor: Binds 1 zinc ion per subunit

By similarity.

Subunit structure: Found in a complex with p53/TP53 and CABLES1. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Interacts with HECW2. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Isoform Beta interacts with WWOX. Interacts (via SAM domain) with FBXO45 (via B30.2/SPRY domain). Interacts with YAP1 (phosphorylated form). Interacts with HCK (via SH3 domain); this inhibits TP73 activity and degradation. Ref.12 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.24 Ref.25 Ref.27

Subcellular location: Nucleus. Cytoplasm. Note: Accumulates in the nucleus in response to DNA damage. Ref.20 Ref.24

Tissue specificity: Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. Ref.6 Ref.21

Induction: Not induced by DNA damage. Isoforms lacking the transactivation domain block gene induction. Ref.6

Domain: Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL1 tyrosine kinase SH3 domain. Ref.19The WW-binding motif mediates interaction with WWOX. Ref.19

Post-translational modification: Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function. Ref.15Higher levels of phosphorylation seen in the brain from patients with Huntington disease.Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome.

Miscellaneous: Maps to a chromosome region frequently mutated in diverse cell lines of human cancer. Appears not to be frequently mutated in human cancers, in contrast to p53/TP53. Hemizygosity is observed in neuroblastoma and oligodendroglioma.Activated and stabilized by interaction with RANBP9.

Sequence similarities: Belongs to the p53 family.Contains 1 SAM (sterile alpha motif) domain.