CD9 / Tspan-29 recombinant protein

Recombinant Human CD9 / Tspan-29 Protein (His tag)

Cat. Num. AAA2545168

• Gene Names: CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CD9 / Tspan-29; Recombinant Human CD9 / Tspan-29 Protein (His tag); MIC3; TSPAN29; GIG2; CD9; CD9 / Tspan-29 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 228

• Application Notes: The recombinant human CD9 consists of 95 amino acids and predictes a molecular mass of 11 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ser 112
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD9 / Tspan-29 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD9 is a member of the transmembrane 4 superfamily, which is also known as the tetraspanin family. CD9 is a cell surface glycoprotein with 4 hydrophobic domains that is described to complex with integrins and other transmembrane 4 superfamily members. It is found expressed on the surface of the exosomes. The protein takes part in cellular signal transduction events and thus play a role in the regulation of cell development and activation, growth and motility. Besides, CD9 seems to be a key role in the egg-sperm fusion during the mammalian fertilization processes. CD9 is found on the membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli.

Description: A DNA sequence encoding the second extracellular domain (Ser 112-Ile 195) of human CD9 (NP_001760.1) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4502693
• NCBI GeneID: 928
• NCBI Accession #: NP_001760.1
• NCBI GenBank Nucleotide #: NM_001769.3
• UniProt Accession #: P21926; Q5J7W6; Q96ES4; D3DUQ9
• Molecular Weight: 25,416 Da
• NCBI Official Full Name: CD9 antigen isoform 1
• NCBI Official Synonym Full Names: CD9 molecule
• NCBI Official Symbol: CD9
• NCBI Official Synonym Symbols: MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• NCBI Protein Information: CD9 antigen
• UniProt Protein Name: CD9 antigen
• UniProt Gene Name: CD9
• UniProt Synonym Gene Names: MIC3; TSPAN29; MRP-1; Tspan-29

NCBI Description

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

Uniprot Description

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular space; focal adhesion; membrane; plasma membrane; platelet alpha granule membrane

Molecular Function: protein binding

Biological Process: cell adhesion; cell motility; cell surface receptor linked signal transduction; fusion of sperm to egg plasma membrane; paranodal junction assembly; platelet degranulation; receptor internalization