Flow Cytometry (FC/FACS)
(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
Mouse anti-Human CD44 Monoclonal Antibody | anti-CD44 antibody
Anti-Human CD44, PE, (clone: BU75)(Mouse IgG2a)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Western Blot, Functional Assay
Synonyms
CD44; Monoclonal Antibody; Anti-Human CD44; PE; (clone: BU75)(Mouse IgG2a); anti-CD44 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
BU75
Specificity
This antibody is specific for human CD44 (Pgp-1)
Form/Format
PE conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5mg/ml.
Applicable Applications for anti-CD44 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Functional Assay (FA)
Application Notes
FC/FACS: 0.2-0.1ug/106cells
IHC, IF, WB, FA: inhibition of CD44?hyaluronate binding
IHC, IF, WB, FA: inhibition of CD44?hyaluronate binding
Conjugation
R-phycoerythrin (PE)
Immunogen
Follicular dendriticcells and germinal center B cells.
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE
Flow Cytometry (FC/FACS)
(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
Flow Cytometry (FC/FACS)
(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
Testing Data
(CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
Testing Data
(CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
Related Product Information for anti-CD44 antibody
The CD44 antigen is a cell-surface glycoprotein involved in cell to cell and cell-matrix interactions, cell adhesion and migration, which it achieves through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction of CD44 is observed innumerous pathogenic phenotypesincluding various forms of cancer.
References
1. Denning, S.M., et al. (1995)in Leucocyte Typing V (Schlossman, S., ed) pp. 1713-1719, Oxford University Press, Oxford.
2. Telen MJ, et al. (1996) A bloodgroup-related polymorphism of CD44 abolishes a hyaluronan-binding consensussequence without preventing hyaluronan binding. J Biol Chem.271(12):7147-53.
3. Zittermann SI, et al.(2001) Modulation of CD44 in acute lymphoblastic leukemia identifies functional andphenotypic differences of human B cell precursors. Eur J Haematol. 66(6):377-82.
2. Telen MJ, et al. (1996) A bloodgroup-related polymorphism of CD44 abolishes a hyaluronan-binding consensussequence without preventing hyaluronan binding. J Biol Chem.271(12):7147-53.
3. Zittermann SI, et al.(2001) Modulation of CD44 in acute lymphoblastic leukemia identifies functional andphenotypic differences of human B cell precursors. Eur J Haematol. 66(6):377-82.
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Product Notes
The CD44 (Catalog #AAA524556) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human CD44, PE, (clone: BU75)(Mouse IgG2a) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD44 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Functional Assay (FA). FC/FACS: 0.2-0.1ug/106cells IHC, IF, WB, FA: inhibition of CD44?hyaluronate binding. Researchers should empirically determine the suitability of the CD44 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD44, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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