Rat anti-Mouse CD117 Monoclonal Antibody | anti-CD117 antibody

Anti-Mouse CD117, PE (Clone 2B8)(rat IgG2b)

Cat. Num. AAA524581

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Purified
• Synonyms: CD117; Monoclonal Antibody; Anti-Mouse CD117; PE (Clone 2B8)(rat IgG2b); anti-CD117 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2b
• Clone Number: 2B8
• Specificity: Mouse CD117 (c-Kit)
• Purity/Purification: Purified
• Form/Format: 50ug in 0.5ml PE-conjugated Ig in phosphate buffered saline containing 0.1% sodium azide (NaN3) as preservative. A highly purified grade of BSA has been added as a stabilizing protein to bring the final protein concentration to 4-5mg/ml after conjugation.
• Concentration: 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD117 antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: FC/FACS: Every lot is tested by flow cytometry using mouse bone marrow suspensions. From this testing, we recommend that between 0.1ug and 0.25ug of antibody be used per 1x106 cells.
• Conjugation: R-phycoerythrin (PE)
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. Avoid prolonged exposure to light. If the reagent is being diluted, it is recommended that only the quantity to be used in one week be diluted.

Flow Cytometry (FC/FACS)

(Balb/c mouse splenic T-cells (left) or thymocytes (right) were stained with anti-CD8a (clone: YTS 169.4)(filled histogram) or rat IgG2b isotype control (open histogram). )

Related Product Information for anti-CD117 antibody

Monoclonal antibody recognizes CD117 (also known as c-Kit), a tyrosine kinase receptor which is expressed on multipotent hematopoietic stem cells, precursors to B cells and T cells, and myeloerythroid progenerators 1,3,8. The interactions of c-Kit and its ligand stem cell factor play a role in the proliferation and differentiation of hematopoietic progenerator cells^1,8.

References

1. Ikuta, K. and Weissman, I.L. 1992. Evidence that hematopoietic stem cells express mouse c-Kit but do not depend on steel factor for their generation. Proc. Nat. Acad. Sci. USA89:1502-1506.
2. Fleming, W.H., Alpern, E.J., Uchida, N., Ikuta, K. and Weissman, I.L. 1993. Steel factor influences the distribution and activity of muring hematopoietic stem cells in vivo. Proc. Natl. Acad. Sci. USA90:3760-3764.
3. Rico-Vargas, S.A., Weiskopf, B., Nishikawa, S.I. and Osmond, D. 1994. c-Kit expression by B cell precursors in mouse bone marrow. J. Immunol.152:2845-2852.
4. Manova, K., Bachvarova, R.F., Huang, E.J., Sanchez, S., Pronovost, S.M., Velazquez, E., McGuire, B., and Besmer, P. 1992. c-kit receptor and ligand expression in postnatal development of the mouse cerebellum suggests a function for c-Kit in inhibitory interneurons. J. Neurosci.12(12):4663-4676.
5. Maeda, H.A., Yamagata, A., Nishikawa, S., Yoshinaga, K., Kobayashi, S., Nishi, K., and Nishikawa, S.I. 1992. Requirement of c-Kit for development of intestinal pacemaker system. Development116:369-375.
6.Takagi, M., Nakahata, T., Kubo, T., Shiohara, M., Koike, K., Miyajima, A., Arai, K.I., Nishikawa, S.I., Zsebo, K.M., and Komiyama, A. 1992. Stimulation of mouse connective tissue-type mast cells by hemopoietic stem cell factor, a ligand for the c-Kit receptor. J. Immunol.148:3446-3453.
7. De Vries, P., Brasel, K.A., McKenna, H.J., Williams, D.E. and Watson, J.D. 1992. Thymus reconstitution by c-Kit-expressing hematopoietic stem cells purified from adult mouse bone marrow. J. Exp. Med.176:1503-1509.
8. Matsuzaki, Y., J.-I Gyotoku, M. Ogawa, S. -i. Nishikawa, Y. Katsura, G. Gachelin, and H. Nakauchi. 1993. Characterization of c-kit positive intrathymic stem cells that are restricted to lymphoid differentiation. J. Exp. Med. 178: 1283-1292.