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Testing Data (Staining of LPS stimulated mouse spleen with MBS633023.)

Rat anti-Mouse CD83 Monoclonal Antibody | anti-CD83 antibody

CD83 (CD83 Antigen, CD83 Molecule, B cell Activation Protein, BL11, Cell Surface Protein HB15, HB15) (FITC)

Gene Names
CD83; BL11; HB15
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
CD83; Monoclonal Antibody; CD83 (CD83 Antigen; CD83 Molecule; B cell Activation Protein; BL11; Cell Surface Protein HB15; HB15) (FITC); Anti -CD83 (CD83 Antigen; anti-CD83 antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9E30
Specificity
Recognizes mouse CD83.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Applicable Applications for anti-CD83 antibody
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul
Hybridoma
Spleen cells from immunized rats was fused with cells of the P3X63.Ag8.653 myeloma cell line.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Testing Data

(Staining of LPS stimulated mouse spleen with MBS633023.)

Testing Data (Staining of LPS stimulated mouse spleen with MBS633023.)

Testing Data

(Staining of LPS stimulated mouse spleen cells with C2435-01.)

Testing Data (Staining of LPS stimulated mouse spleen cells with C2435-01.)
Related Product Information for anti-CD83 antibody
CD83, a 45kD type 1 glyoprotein belonging to the immunoglobulin superfamily. Originally described as a maturation marker for dendritic cells, CD83 plays a role in the regulation of antigen presentation, thymic T-cell maturation, B-cell function and peripheral T-cell activation. Activated dendritic cells and B-cells release a soluble form of CD83 that is reported to have an immunosuppressive effect, suggesting a therapeutic role for sCD83 in the regulation of immune disorders.
Product Categories/Family for anti-CD83 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,042 Da
NCBI Official Full Name
CD83 antigen isoform a
NCBI Official Synonym Full Names
CD83 molecule
NCBI Official Symbol
CD83
NCBI Official Synonym Symbols
BL11; HB15
NCBI Protein Information
CD83 antigen; hCD83; OTTHUMP00000016057; B-cell activation protein; cell surface protein HB15; cell-surface glycoprotein; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)
UniProt Protein Name
CD83 antigen
Protein Family
UniProt Gene Name
CD83
UniProt Entry Name
CD83_HUMAN

NCBI Description

The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

CD83: May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Biological Process: negative regulation of interleukin-4 production; positive regulation of interleukin-2 production; defense response; positive regulation of CD4-positive, alpha beta T cell differentiation; signal transduction; response to organic cyclic substance; positive regulation of interleukin-10 production; humoral immune response

Research Articles on CD83

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Product Notes

The CD83 cd83 (Catalog #AAA633023) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD83 (CD83 Antigen, CD83 Molecule, B cell Activation Protein, BL11, Cell Surface Protein HB15, HB15) (FITC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD83 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul. Researchers should empirically determine the suitability of the CD83 cd83 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD83, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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