Rabbit anti-Human, Mouse BHLHA15 Polyclonal Antibody | anti-BHLHA15 antibody

BHLHA15, CT (BHLHA15, BHLHB8, MIST1, Class A basic helix-loop-helix protein 15, Class B basic helix-loop-helix protein 8, Muscle, intestine and stomach expression 1) (APC)

Cat. Num. AAA6278341

• Gene Names: BHLHA15; MIST1; BHLHB8
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: BHLHA15; Polyclonal Antibody; CT (BHLHA15; BHLHB8; MIST1; Class A basic helix-loop-helix protein 15; Class B basic helix-loop-helix protein 8; Muscle; intestine and stomach expression 1) (APC); intestine and stomach expression 1; anti-BHLHA15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BHLHA15. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-BHLHA15 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 160-189 in the C-terminal region of human BHLHA15, UniProt Accession #Q7RTS1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in U251 cell line lysates (35ug/lane) usingMBS6012885. This demonstrates that MBS6012885 detected the BHLHA15 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse Neuro-2a cell line lysates (35ug/lane) usingMBS6012885. This demonstrates that MBS6012885 detected the BHLHA15 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of U251 cells using MBS6012885 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-BHLHA15 antibody

Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3' (By similarity).

Product Categories/Family for anti-BHLHA15 antibody

Antibodies; Transcription Factors

References

1. Smits, K.M., et al. Ann Epidemiol 20(5):401-404(2010). 2. Tian, X., et al. Mol. Cell. Biol. 30(5):1269-1284(2010). 3. Stevens, J.D., et al. Differentiation 76(9):1006-1022(2008). 4. Zhao, Y., et al. Mol. Endocrinol. 20(9):2187-2198(2006). 5. McLellan, A.S., et al. Mech. Dev. 119 SUPPL 1, S285-S291 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 29126247
• NCBI GeneID: 168620
• NCBI Accession #: NP_803238.1
• NCBI GenBank Nucleotide #: NM_177455.3
• UniProt Accession #: Q7RTS1; Q14DE4; A4D271
• Molecular Weight: 20,818 Da
• NCBI Official Full Name: class A basic helix-loop-helix protein 15
• NCBI Official Synonym Full Names: basic helix-loop-helix family, member a15
• NCBI Official Symbol: BHLHA15
• NCBI Official Synonym Symbols: MIST1; BHLHB8
• NCBI Protein Information: class A basic helix-loop-helix protein 15; MIST-1; class II bHLH protein MIST1; class B basic helix-loop-helix protein 8; muscle, intestine and stomach expression 1; basic helix-loop-helix domain containing, class B, 8
• UniProt Protein Name: Class A basic helix-loop-helix protein 15
• Protein Family: Class A basic helix-loop-helix protein
• UniProt Gene Name: BHLHA15
• UniProt Synonym Gene Names: BHLHB8; MIST1; bHLHa15; bHLHb8; MIST-1
• UniProt Entry Name: BHA15_HUMAN

Uniprot Description

MIST1: Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E- box occupancy by homodimers. May also negatively regulate bHLH- mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E- box consensus sequence 5'-CANNTG-3'.

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: nucleus

Molecular Function: protein homodimerization activity

Biological Process: G-protein coupled receptor protein signaling pathway; cellular response to glucose starvation; transcription, DNA-dependent; cell-cell signaling; unfolded protein response; cell maturation; intracellular distribution of mitochondria; calcium-mediated signaling; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis; Golgi organization and biogenesis; mitochondrial calcium ion transport