Testing Data
Mouse anti-Human CD80 Monoclonal Antibody | anti-CD80 antibody
anti-human CD80 (B7-1)-Purified (with Antibiotics)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified (with Antibiotics)
Synonyms
CD80; Monoclonal Antibody; anti-human CD80 (B7-1)-Purified (with Antibiotics); Monoclonal anti-human CD80 (B7-1) (IgG1 isotype); anti-CD80 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
P1.H1.A1.A1
Purity/Purification
Purified (with Antibiotics)
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
316
Applicable Applications for anti-CD80 antibody
Flow Cytometry (FC/FACS), ELISA (EIA)
Performance
Five x 105 cultured human Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD80 at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
Group Header
anti-human CD80 (B7-1)
Immunogen
Human CD80 (B7-1) Ig Fusion Protein
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD80 antibody
Information: Human CD80 (B7-1) is a costimulatory ligand for CD28 and CTLA-4. CD80 is expressed on activated B cells. Antibody P1.H5.A1.A1 recognizes the CD80 molecule and blocks binding of soluble CD152 Ig fusion protein to CD80.
References
C.B. Thompson, (1995) Cell 81: 979-982. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 682-684
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD80 molecule
NCBI Official Synonym Full Names
CD80 molecule
NCBI Official Symbol
CD80
NCBI Protein Information
CD80 molecule; CD80 antigen
Protein Family
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Product Notes
The CD80 (Catalog #AAA666674) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD80 (B7-1)-Purified (with Antibiotics) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD80 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the CD80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD80, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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