Testing Data
Mouse anti-Human CD278 Monoclonal Antibody | anti-CD278 antibody
anti-human CD278(ICOS)-Purified (with Antibiotics)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified (with Antibiotics)
Synonyms
CD278; Monoclonal Antibody; anti-human CD278(ICOS)-Purified (with Antibiotics); Monoclonal anti-human CD278 (ICOS); anti-CD278 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
ANC6C6
Purity/Purification
Purified (with Antibiotics)
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-CD278 antibody
Flow Cytometry (FC/FACS), ELISA (EIA)
Performance
Five x 105 human HPB-MLT cells were washed incubated 45 minutes on ice with 80 ul of anti-human ICOS antibody at 1 ug/ml. Cells were washed twice and incubated with Goat-anti-Mouse/FITC, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.63 log10 fluorescent units when compared to isotype control Mouse IgG1 antibody.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
Group Header
anti-human CD278(ICOS)
Immunogen
Human HPB-MLT cells, recombinant human CD278(ICOS)-muIg
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD278 antibody
Information: The inducible costimulator CD278 (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells.1 Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement.2 Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS.3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain. 4
Antibody ANC6C6 binds to recombinant ICOS-muIg in EIA, and to the surface of HPB-MLT cells in Flow cytometry. Additionally, it blocks binding of recombinant GL50-muIg to HPB-MLT cells.
References
1) Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717. 2) Riley, J.L., C.H. June, et al. 2001, J. Immunol. 166: 4943-4948. 3) Ling, V., M. Collins, et al. 2000, J. Immunol. 164: 1653-1657. 4) Ling, V., M. Collins, et al. 2001, J. Immunol. 166: 7300-7308.
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Product Notes
The CD278 (Catalog #AAA666142) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD278(ICOS)-Purified (with Antibiotics) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD278 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the CD278 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD278, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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