Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with CD8a antibody (biotin) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
Mouse anti-Human CD8 Monoclonal Antibody | anti-CD8 antibody
CD8 antibody (biotin)
Gene Names
CD8A; CD8
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD8; Monoclonal Antibody; CD8 antibody (biotin); Mouse monoclonal CD8 antibody (biotin); Monoclonal CD8 antibody; Anti-CD8 antibody; CD-8; CD 8; CD-8 antibody; CD8 alpha antibody; CD 8 antibody; leu-2a antibody; anti-CD8 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
HIT8a
Specificity
Human
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
20 ul (0.25 ug)/test (varies by lot)
Sequence Length
216
Applicable Applications for anti-CD8 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: 20 ul (0.25 ug)/test
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test
Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with CD8a antibody (biotin) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with CD8a antibody (biotin) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
Related Product Information for anti-CD8 antibody
The HIT8a monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.
Product Categories/Family for anti-CD8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
24,053 Da
NCBI Official Full Name
CD8, partial
NCBI Official Synonym Full Names
CD8a molecule
NCBI Official Symbol
CD8A
NCBI Official Synonym Symbols
CD8
NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain
UniProt Protein Name
CD8
Protein Family
Research Articles on CD8
Similar Products
Product Notes
The CD8 (Catalog #AAA5309399) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD8 antibody (biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: 20 ul (0.25 ug)/test A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. Researchers should empirically determine the suitability of the CD8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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