Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with 0.125 ug of Rat IgG2b kappa Isotype Control (biotin) (open histogram) or 0.125 ug of CD4 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Rat anti-Mouse CD4 Monoclonal Antibody | anti-CD4 antibody
CD4 antibody (biotin)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
CD4; Monoclonal Antibody; CD4 antibody (biotin); Rat monoclonal CD4 antibody (biotin); Monoclonal CD4 antibody; Anti-CD4 antibody; L3T4 antibody; Ly-4 antibody; CD-4 antibody; CD 4; CD-4; CD 4 antibody; anti-CD4 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
GK1.5
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
20 ul (0.2 ug)/test (varies by lot)
Sequence Length
487
Applicable Applications for anti-CD4 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: 20 ul (0.2 ug)/test
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test
Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with 0.125 ug of Rat IgG2b kappa Isotype Control (biotin) (open histogram) or 0.125 ug of CD4 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with 0.125 ug of Rat IgG2b kappa Isotype Control (biotin) (open histogram) or 0.125 ug of CD4 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Related Product Information for anti-CD4 antibody
The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.
Product Categories/Family for anti-CD4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
54,985 Da
NCBI Official Full Name
CD4
NCBI Official Synonym Full Names
CD4 molecule
NCBI Official Symbol
CD4
NCBI Protein Information
T-cell surface glycoprotein CD4
UniProt Protein Name
CD4 protein
Protein Family
UniProt Gene Name
CD4
Research Articles on CD4
Similar Products
Product Notes
The CD4 cd4 (Catalog #AAA5309392) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD4 antibody (biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: 20 ul (0.2 ug)/test A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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