Rabbit anti-Human NMI Polyclonal Antibody | anti-NMI antibody

NMI, NT (NMI, N-myc-interactor, N-myc and STAT interactor) (PE)

Cat. Num. AAA6325861

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NMI; Polyclonal Antibody; NT (NMI; N-myc-interactor; N-myc and STAT interactor) (PE); N-myc and STAT interactor; anti-NMI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NMI antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NMI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NMI.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NMI Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the NMI antibody detected the NMI protein (arrow).)

Related Product Information for anti-NMI antibody

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq].

Product Categories/Family for anti-NMI antibody

Antibodies; Transcription Factors; STAT

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Fillmore, R.A., et al. Int. J. Cancer 125(3):556-564(2009). Quaye, L., et al. Br. J. Cancer 100(6):993-1001(2009). Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009). Quaye, L., et al. Clin. Cancer Res. 14(18):5833-5839(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 188219599
• NCBI GeneID: 9111
• NCBI Accession #: NP_004679.2
• NCBI GenBank Nucleotide #: NM_004688.2
• UniProt Accession #: Q13287; Q53TI8; Q9BVE5; B5BU69
• Molecular Weight: 35,057 Da
• NCBI Official Full Name: N-myc-interactor
• NCBI Official Synonym Full Names: N-myc (and STAT) interactor
• NCBI Official Symbol: NMI
• NCBI Protein Information: N-myc-interactor; N-myc interactor
• UniProt Protein Name: N-myc-interactor
• Protein Family: N-myc-interactor
• UniProt Gene Name: NMI
• UniProt Synonym Gene Names: Nmi
• UniProt Entry Name: NMI_HUMAN

NCBI Description

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008]

Uniprot Description

NMI: May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5. Belongs to the NMI family.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; transcription cofactor activity

Biological Process: transcription from RNA polymerase II promoter; inflammatory response; JAK-STAT cascade