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Western Blot (WB) (Western blot analysis of GALT Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). GALT (arrow) was detected using the purified Pab.)

Rabbit anti-Human Galactose-1-phosphate Uridylyltransferase Polyclonal Antibody | anti-GALT antibody

Galactose-1-phosphate Uridylyltransferase, CT (Gal-1-P Uridylyltransferase, GALT, UDP-glucose-hexose-1-phosphate Uridylyltransferase) (Azide free) (HRP)

Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Galactose-1-phosphate Uridylyltransferase; Polyclonal Antibody; CT (Gal-1-P Uridylyltransferase; GALT; UDP-glucose-hexose-1-phosphate Uridylyltransferase) (Azide free) (HRP); Galactose-1-phosphate uridylyltransferase; Gal-1-P uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; anti-GALT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GALT.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-GALT antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human GALT (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GALT Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). GALT (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of GALT Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). GALT (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with GALT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human breast carcinoma reacted with GALT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-MB468 cells using GALT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of MDA-MB468 cells using GALT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-GALT antibody
References
1. Leslie, N.D., et.al., Genomics 14(2), 474-480 (1992).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
galactose-1-phosphate uridylyltransferase isoform 1
NCBI Official Synonym Full Names
galactose-1-phosphate uridylyltransferase
NCBI Official Symbol
GALT
NCBI Protein Information
galactose-1-phosphate uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; gal-1-P uridylyltransferase; galactose-1-phosphate uridyl transferase
UniProt Protein Name
Galactose-1-phosphate uridylyltransferase
Protein Family
UniProt Gene Name
GALT
UniProt Synonym Gene Names
Gal-1-P uridylyltransferase
UniProt Entry Name
GALT_HUMAN

NCBI Description

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

GALT: Defects in GALT are the cause of galactosemia (GALCT). Galactosemia is an inherited disorder of galactose metabolism that causes jaundice, cataracts, and mental retardation. Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.

Protein type: Motility/polarity/chemotaxis; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 2.7.7.12; Carbohydrate Metabolism - galactose; Transferase

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: Golgi apparatus; cytosol

Molecular Function: zinc ion binding; UDP-glucose:hexose-1-phosphate uridylyltransferase activity

Biological Process: galactose catabolic process; carbohydrate metabolic process; UDP-glucose catabolic process; galactose metabolic process; pathogenesis

Disease: Galactosemia

Research Articles on GALT

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Product Notes

The GALT galt (Catalog #AAA6477430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Galactose-1-phosphate Uridylyltransferase, CT (Gal-1-P Uridylyltransferase, GALT, UDP-glucose-hexose-1-phosphate Uridylyltransferase) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Galactose-1-phosphate Uridylyltransferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GALT galt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Galactose-1-phosphate Uridylyltransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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