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Western Blot (WB)
(Western blot analysis of CD8 alpha on different lysates using anti-CD8 alpha antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Lovo)
Rabbit anti-Human CD8 alpha Monoclonal Antibody | anti-CD8A antibody
CD8 alpha Antibody
Gene Names
CD8A; CD8; MAL; p32; Leu2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
CD8 alpha; Monoclonal Antibody; CD8 alpha Antibody; alpha polypeptide (p32) antibody; CD8 antibody; CD8 antigen alpha polypeptide antibody; CD8 antigen alpha polypeptide (p32) antibody; CD8a antibody; CD8A antigen antibody; CD8A molecule antibody; CD8A_HUMAN antibody; Leu2 antibody; Leu2 T lymphocyte antigen antibody; Ly3 antibody; LYT3 antibody; MAL antibody; OKT8 T cell antigen antibody; OTTHUMP00000160760 antibody; OTTHUMP00000160764 antibody; OTTHUMP00000203528 antibody; OTTHUMP00000203721 antibody; p32 antibody; T cell antigen Leu2 antibody; T cell co receptor antibody; T-cell surface glycoprotein CD8 alpha chain antibody; T-lymphocyte differentiation antigen T8/Leu-2 antibody; T8 T cell antigen antibody; T8/Leu-2 T-lymphocyte differentiation antigen antibody; anti-CD8A antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
SI18-01
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CD8A antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of CD8 alpha on different lysates using anti-CD8 alpha antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Lovo)
Western Blot (WB)
(Western blot analysis of CD8 alpha on different lysates using anti-CD8 alpha antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Lovo)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD8 alpha antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD8 alpha antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining CD8 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining CD8 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining CD8 alpha in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining CD8 alpha in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-CD8A antibody
The T cell receptor (TCR) is a heterodimer composed of either alpha and beta or gamma and delta chains. CD3 chains and the CD4 or CD8 (CD8-alpha and CD8-beta ) co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8 proteins; T helper cells express CD4 proteins and T cytotoxic cells display CD8 proteins. CD8s are cell surface glycoproteins that exist as two chain complex (alphaalpha or alphabeta ) receptors that bind class I MHC molecules presented by the antigen-presenting cell (APC). A primary function of CD8 proteins is to facilitate antigen recognition by the TCR and to strengthen the avidity of the TCR-antigen interactions. An additional role for CD8-expressing T cells may be to maintain low levels of HIV expression.
Product Categories/Family for anti-CD8A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32kDa
NCBI Official Full Name
T-cell surface glycoprotein CD8 alpha chain isoform 1
NCBI Official Synonym Full Names
CD8a molecule
NCBI Official Symbol
CD8A
NCBI Official Synonym Symbols
CD8; MAL; p32; Leu2
NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
Protein Family
UniProt Gene Name
CD8A
UniProt Synonym Gene Names
MAL
UniProt Entry Name
CD8A_HUMAN
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Product Notes
The CD8A cd8a (Catalog #AAA9435583) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD8 alpha Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the CD8A cd8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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