Western Blot (WB)
(Anti- CD 8 alpha Picoband antibody, MBS178342, Western blottingAll lanes: Anti CD 8 alpha (MBS178342) at 0.5ug/mlWB: Recombinant Human CD 8 alpha Protein 0.5ngPredicted bind size: 29KDObserved bind size: 29KD )
anti-Human CD8 alpha Polyclonal Antibody | anti-CD8 alpha antibody
Anti-CD8 alpha Antibody
Gene Names
CD8A; CD8; MAL; p32; Leu2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Synonyms
CD8 alpha; Polyclonal Antibody; Anti-CD8 alpha Antibody; T-cell surface glycoprotein CD8 alpha chain; alpha polypeptide (p32); CD 8a; CD8 alpha chain; CD8; CD8 antigen alpha chain; CD8 antigen alpha polypeptide; CD8 antigen alpha polypeptide (p32); CD8a; CD8a antigen; CD8a molecule; CD8A_HUMAN; Leu 2; Leu2; Leu2 T lymphocyte antigen; Ly3; LYT3; MAL; OKT8 T cell antigen; OTTHUMP00000160760; OTTHUMP00000160764; OTTHUMP00000203528; OTTHUMP00000203721; p32; T cell antigen Leu2; T cell co receptor; T cell coreceptor; T cell surface glycoprotein CD8 alpha chain; T lymphocyte differentiation antigen T8/Leu 2; T lymphocyte differentiation antigen T8/Leu2; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen; T8/Leu-2 T-lymphocyte differentiation antigen; anti-CD8 alpha antibody
Reactivity
Human
Clonality
Polyclonal
Specificity
No Cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
235
Applicable Applications for anti-CD8 alpha antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
Western Blot
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu
Antigen Retrieval: By Heat
WB: The detection limit for CD8 alpha is approximately 0.2ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu
Antigen Retrieval: By Heat
WB: The detection limit for CD8 alpha is approximately 0.2ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
E Coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti- CD 8 alpha Picoband antibody, MBS178342, Western blottingAll lanes: Anti CD 8 alpha (MBS178342) at 0.5ug/mlWB: Recombinant Human CD 8 alpha Protein 0.5ngPredicted bind size: 29KDObserved bind size: 29KD )
Western Blot (WB)
(Anti- CD 8 alpha Picoband antibody, MBS178342, Western blottingAll lanes: Anti CD 8 alpha (MBS178342) at 0.5ug/mlWB: Recombinant Human CD 8 alpha Protein 0.5ngPredicted bind size: 29KDObserved bind size: 29KD )
Western Blot (WB)
(Anti- CD 8 alpha Picoband antibody, MBS178342, Western blottingAll lanes: Anti CD 8 alpha (MBS178342) at 0.5ug/mlLane 1: JURKAT Whole Cell Lysate at 40ugLane 2: RAJI Whole Cell Lysate at 40ugLane 3: HL-60 Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugPredicted bind size: 26KDObserved bind size: 55KD )
Western Blot (WB)
(Anti- CD 8 alpha Picoband antibody, MBS178342, Western blottingAll lanes: Anti CD 8 alpha (MBS178342) at 0.5ug/mlLane 1: JURKAT Whole Cell Lysate at 40ugLane 2: RAJI Whole Cell Lysate at 40ugLane 3: HL-60 Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugPredicted bind size: 26KDObserved bind size: 55KD )
Immunohistochemistry (IHC)
(Anti- CD 8 alpha Picoband antibody, MBS178342, IHC(P)IHC(P): Human Tonsil Tissue )
Immunohistochemistry (IHC)
(Anti- CD 8 alpha Picoband antibody, MBS178342, IHC(P)IHC(P): Human Tonsil Tissue )
Related Product Information for anti-CD8 alpha antibody
Description: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD8 alpha chain(CD8A) detection. Tested with WB, IHC-P in Human.
Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
References
1. Bowcock, A. M., Kavathas, P., Margolskee, R. F., Herzenberg, L., Cavalli-Sforza, L. L. An RFLP associated with pcDLeu2-14, a human T-cell differentiation antigen CD8 (Leu2) cDNA mapped to 2p12. Nucleic Acids Res. 14: 7817 only, 1986. 2. "Entrez Gene: CD8A CD8a molecule"
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,214 Da
NCBI Official Full Name
T-cell surface glycoprotein CD8 alpha chain isoform 1
NCBI Official Synonym Full Names
CD8a molecule
NCBI Official Symbol
CD8A
NCBI Official Synonym Symbols
CD8; MAL; p32; Leu2
NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
UniProt Gene Name
CD8A
UniProt Synonym Gene Names
MAL
UniProt Entry Name
CD8A_HUMAN
NCBI Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Research Articles on CD8 alpha
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Product Notes
The CD8 alpha cd8a (Catalog #AAA178342) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD8 alpha Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot Concentration: 0.1-0.5ug/ml Tested Species: Hu Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1ug/ml Tested Species: Hu Antigen Retrieval: By Heat WB: The detection limit for CD8 alpha is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the CD8 alpha cd8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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