Mouse anti-Human CD74 Monoclonal Antibody | anti-CD74 antibody

anti-human CD74-R-PE

Cat. Num. AAA666206

• Gene Names: CD74; II; DHLAG; HLADG; Ia-GAMMA
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD74; Monoclonal Antibody; anti-human CD74-R-PE; Monoclonal anti-human CD74/R-PE; anti-CD74 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: M-B741
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
• Concentration: Volume: 0.2 ml (varies by lot)
• Sequence Length: 160

• Applicable Applications for anti-CD74 antibody: Flow Cytometry (FC/FACS)
• Application Notes: 1:50 (or use 1.6mul of concentrated stock per 5 x 105-cell test)
• Performance: Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 mul of anti-CD74/R-PE at a 1:50 dilution (10 mug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.35 log10 fluorescent units when compared to a Mouse IgG2a/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated with 20 mul of 0.5 mg/ml unlabeled anti-CD74 antibody.
• Conjugation: PE
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant, and conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A565/A280 ratio of 2.6.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: RPMI 1788 cell line
• Preparation and Storage: Store at 2 - 5 degree C. Do not freeze! Protect from light.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD74 antibody

Information: Human CD74 is expressed intracellularly and on the cell surface of MHC Class II positive cells such as B cells, monocytes, macrophages, Langerhans cells and dendritic cells. CD74 has several important functions relating to antigen presentation by MHC Class II molecules. Antibody M-B741 recognizes the CD74 molecule of about 33 kd.

References

Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 568-574. P. Romagnoli & R.N. Germain, (1995) J Exp Med 182: 2027-2036.

NCBI and Uniprot Product Information

• NCBI GI #: 18999403
• NCBI GeneID: 972
• Molecular Weight: 18,328 Da
• NCBI Official Full Name: CD74 molecule, major histocompatibility complex, class II invariant chain
• NCBI Official Synonym Full Names: CD74 molecule, major histocompatibility complex, class II invariant chain
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
• UniProt Protein Name: HLA class II histocompatibility antigen gamma chain
• Protein Family: H-2 class II histocompatibility antigen
• UniProt Gene Name: CD74
• UniProt Synonym Gene Names: DHLAG; Ii
• UniProt Entry Name: HG2A_HUMAN

NCBI Description

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

CD74: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74- ROS1 chimeric protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: Golgi membrane; multivesicular body; lysosomal lumen; cell surface; membrane; lysosomal membrane; plasma membrane; integral to membrane; trans-Golgi network membrane; vacuole; intracellular; external side of plasma membrane; MHC class II protein complex

Molecular Function: identical protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; beta-amyloid binding; cytokine binding; MHC class II protein binding, via antigen binding groove; nitric-oxide synthase binding; MHC class II protein binding

Biological Process: activation of MAPK activity; positive regulation of dendritic cell antigen processing and presentation; antigen processing and presentation of exogenous peptide antigen via MHC class II; defense response; signal transduction; prostaglandin biosynthetic process; negative regulation of T cell differentiation; intracellular protein transport; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of fibroblast proliferation; negative regulation of mature B cell apoptosis; positive regulation of B cell proliferation; protein complex assembly; positive thymic T cell selection; negative regulation of peptide secretion; positive regulation of T-helper 2 type immune response; chaperone cofactor-dependent protein folding; regulation of macrophage activation; T cell selection; negative thymic T cell selection; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell differentiation; immunoglobulin mediated immune response; positive regulation of cytokine and chemokine mediated signaling pathway; antigen processing and presentation of endogenous antigen; negative regulation of apoptosis