Flow Cytometry (FC/FACS)
(CD69 Purified mAb Staining of overnight PHA-stimulated normal human peripheral blood cells, followed by anti-mouse IgGs-PE)
Mouse anti-Human CD69 Monoclonal Antibody | anti-CD69 antibody
Mouse anti-Human CD69, Purified mAb
Gene Names
CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
CD69; Monoclonal Antibody; Mouse anti-Human CD69; Purified mAb; anti-CD69 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
4AFN50
Form/Format
Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Sequence Length
21
Applicable Applications for anti-CD69 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Experimental Methods
1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7.Repeat step 5.
8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7.Repeat step 5.
8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
Flow Cytometry (FC/FACS)
(CD69 Purified mAb Staining of overnight PHA-stimulated normal human peripheral blood cells, followed by anti-mouse IgGs-PE)
Flow Cytometry (FC/FACS)
(CD69 Purified mAb Staining of overnight PHA-stimulated normal human peripheral blood cells, followed by anti-mouse IgGs-PE)
Related Product Information for anti-CD69 antibody
The 4AFN50 monoclonal antibody reacts with human CD69, also known as very earlyactivation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.
References
1.Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York. 2.Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
2,282 Da
NCBI Official Full Name
CD69, partial
NCBI Official Synonym Full Names
CD69 molecule
NCBI Official Symbol
CD69
NCBI Official Synonym Symbols
AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
NCBI Protein Information
early activation antigen CD69
UniProt Protein Name
CD69 protein
Protein Family
UniProt Gene Name
CD69
NCBI Description
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]
Research Articles on CD69
Similar Products
Product Notes
The CD69 cd69 (Catalog #AAA8510529) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Human CD69, Purified mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD69 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the CD69 cd69 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD69, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
