Rat CD68 Monoclonal Antibody | anti-CD68 antibody
RAT ANTI MOUSE CD68:FITC
Gene Names
Cd68; Lamp4; gp110; Scard1
Applications
Flow Cytometry, Functional Assay
Synonyms
CD68; Monoclonal Antibody; RAT ANTI MOUSE CD68:FITC; MACROSIALIN; anti-CD68 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
FA-11
Specificity
Rat anti Mouse CD68 antibody, clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.
CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.
CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used in cancer studies to demonstrate the presence/localization of macrophages.
Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues (Masaki et al. 2003) and (Devey et al. 2009).
Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.
CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used in cancer studies to demonstrate the presence/localization of macrophages.
Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues (Masaki et al. 2003) and (Devey et al. 2009).
Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Form/Format
FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 - liquid
Concentration
Approx. IgG concentration - 0.1mg/ml (varies by lot)
Sequence Length
318
Applicable Applications for anti-CD68 antibody
Flow cytometry (FC/FACS)*
Application Notes
Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm™ (MBS225936) for this purpose.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR
(MBS238295 or MBS238296).
Flow Cytometry - Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR
(MBS238295 or MBS238296).
Flow Cytometry - Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
1% Bovine Serum Albumin
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Purified Concanavalin A acceptor glycoprotein from P815 cell line
Max Ex/Em
FLuorophore - FITC
Excitation Max (nm) - 490
Emission Max (nm) - 525
Excitation Max (nm) - 490
Emission Max (nm) - 525
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Preparation and Storage
This product is shipped at ambient temperature.
It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed and keep aliquots at 2-8°C for short term use (up to 4 weeks). Store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed and keep aliquots at 2-8°C for short term use (up to 4 weeks). Store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Synonym Full Names
CD68 antigen
NCBI Official Symbol
Cd68
NCBI Official Synonym Symbols
Lamp4; gp110; Scard1
NCBI Protein Information
macrosialin
UniProt Protein Name
Macrosialin
Protein Family
UniProt Gene Name
Cd68
UniProt Entry Name
CD68_MOUSE
Uniprot Description
CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell surface; Membrane protein, integral
Cellular Component: membrane; lysosome; plasma membrane; integral to membrane; endosome
Research Articles on CD68
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Product Notes
The CD68 cd68 (Catalog #AAA210809) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD68 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS)*. Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm™ (MBS225936) for this purpose. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (MBS238295 or MBS238296). Flow Cytometry - Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the CD68 cd68 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD68, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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