Testing Data #1
(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:Biotin (MBS215398).following permeablisation with Leucoperm )
Rat CD68 Monoclonal Antibody | anti-CD68 antibody
RAT ANTI MOUSE CD68:Endotoxin Low
Purified IgG - liquid
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilization is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Immunohistology - Paraffin: Application Note: This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Martin-Manso for details. Staining has also been achieved without antigen retrieval, see Lu for details.
Western Blotting: Application Note: Non-reducing conditions recommended.
Buffer Solution
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:Biotin (MBS215398).following permeablisation with Leucoperm )
Testing Data #1
(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:Biotin (MBS215398).following permeablisation with Leucoperm )
Testing Data #2
(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:FITC (MBS215400). following permeablisation with Leucoperm )
Testing Data #2
(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:FITC (MBS215400). following permeablisation with Leucoperm )
Testing Data #3
(Staining of permeabilised mouse peritoneal macrophages cells with Rat anti Mouse CD68 (MBS215395) visualised with F(ab')2 Goat anti Rat IgG:FITC (Mouse adsorbed) (MBS235148))
Testing Data #3
(Staining of permeabilised mouse peritoneal macrophages cells with Rat anti Mouse CD68 (MBS215395) visualised with F(ab')2 Goat anti Rat IgG:FITC (Mouse adsorbed) (MBS235148))
Testing Data #4
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD68 CD68 (MBS215403), following permeablisation with Leucoperm )
Testing Data #4
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD68 CD68 (MBS215403), following permeablisation with Leucoperm )
Testing Data #5
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa FluorR 488 (MBS215395). following permeablisation with Leucoperm )
Testing Data #5
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa FluorR 488 (MBS215395). following permeablisation with Leucoperm )
Testing Data #6
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: RPE (MBS215405). following permeablisation with Leucoperm )
Testing Data #6
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: RPE (MBS215405). following permeablisation with Leucoperm )
Testing Data #7
(Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa FluorR 647 (MBS215395). following permeablisation with Leucoperm )
Testing Data #7
(Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa FluorR 647 (MBS215395). following permeablisation with Leucoperm )
Testing Data #8
(Staining of mouse spleen cryosection with Rat anti Mouse CD68 (MBS215395) followed by Goat anti Rat IgG:HRP (MBS220363) showing staining of macrophages in the red pulp)
Testing Data #8
(Staining of mouse spleen cryosection with Rat anti Mouse CD68 (MBS215395) followed by Goat anti Rat IgG:HRP (MBS220363) showing staining of macrophages in the red pulp)
Testing Data #9
(Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 (MBS215403) with Goat anti Rat IgG:HRP MBS235195) as a detection antibody)
Testing Data #9
(Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 (MBS215403) with Goat anti Rat IgG:HRP MBS235195) as a detection antibody)
NCBI and Uniprot Product Information
Uniprot Description
CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell surface; Membrane protein, integral
Cellular Component: membrane; lysosome; integral to membrane; plasma membrane; endosome
Research Articles on CD68
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Product Notes
The CD68 cd68 (Catalog #AAA224483) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD68 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)*. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilization is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. Immunohistology - Paraffin: Application Note: This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Martin-Manso for details. Staining has also been achieved without antigen retrieval, see Lu for details. Western Blotting: Application Note: Non-reducing conditions recommended. Researchers should empirically determine the suitability of the CD68 cd68 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD68, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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