Mouse anti-Human CD66b Monoclonal Antibody | anti-CD66b antibody

Monoclonal anti-human CD66b (CEACAM8)/R-PE

Cat. Num. AAA666950

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA
• Synonyms: CD66b; Monoclonal Antibody; Monoclonal anti-human CD66b (CEACAM8)/R-PE; anti-CD66b (ANC1D5); anti-human CD66b (CEACAM8); CEACAM8; CGMb; NCA-95; CD67; anti-CD66b antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: ANC1D5
• Concentration: Volume in Vial: 0.2 ml (varies by lot)

• Applicable Applications for anti-CD66b antibody: Flow Cytometry (FC/FACS), ELISA (EIA) (detect)
• Application Notes: Working Dilution: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
• Immunogen: Recombinant soluble human CD66b
• Conjugation: R-PE
• Performance: Five x 105 Polymorphoprep prepared human Granulocytes (PMN) per tube were pelleted and preincubated 5 minutes with 20ul of human IgG at 300ug/ml (to reduce nonspecific binding) after which they were washed and incubated 45 minutes on ice with 80ul of anti-CD66b (ANC1D5)/PE at a 1:50 dilution (10ug/ml). They were then washed three times, fixed andanalyzed by FACS. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to a Mouse IgG1/PE negative control (#MBS666704). Binding was blocked when cells were preincubated 10 minutes with 20ul of 0.5mg/ml anti CD66b (ANC1D5) antibody (#MBS666894).
• Preparation and Storage: Store at 2-5 degree C. Do not freeze! Protect from light.; Product should retain activity for at least 12 months after shipping date when stored asrecommended.; 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04%NaN3 (as a preservative).; Protein A purified antibody from tissue culture supernatant wasconjugated to R-Phycoerythrin through a sulfo-ester linkage.Unconjugated antibody was removed using size exclusionchromatography. Antibody conjugate is at 500ug/ml with anA565/A280 ratio of 2.83.

Testing Data

Related Product Information for anti-CD66b antibody

Human CD66b (CEACAM8, CGMb, NCA-95, CD67) is a 95kD member of the Carcinoembryonic Antigen (CEA) group. It is expressed on granulocytes and functions as an adhesion molecule. Similar to the CD66 a, c, and d isoforms, engagement of CD66b on neutrophils causes increased adhesion to endothelial cells (2). Crosslinking CD66b on neutrophils with antibodies triggers IL-8 release (3).
Antibody from clone ANC1D5 binds to CD66b on the cell surface of human PMN in Flow cytometry and detects recombinant CD66b in sandwich EIA utilizing either clone ANC4A4 (#MBS666902) or ANC3C5 (#MBS666891) as a capture reagent. Clone ANC1D5 may cross react with other CD66 isoforms, as it also binds to cell surface of an epithelial tumor line.

References

1) W.N. Khan, S. Hammarstrom, et al. (1992) Genomics 14 (2):384-90.
2) K.M. Skubitz, A.P. Skubitz, et al. (1996) J Leuk Biol 60 (1): 106-117.
3) A.K. Schroder, L Rink, et al. (2006) Human Immunol 67 (9): 676-682.