Mouse anti-Human CD66b Monoclonal Antibody | anti-CEACAM8 antibody

Anti-CD66b Antibody (FITC), Mouse Monoclonal

Cat. Num. AAA8105749

• Gene Names: CEACAM8; CD67; CGM6; CD66b; NCA-95
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD66b; Monoclonal Antibody; Anti-CD66b Antibody (FITC); Mouse Monoclonal; CEACAM8/CD66b/CD67 Antibody (FITC); Mouse MAb; carcinoembryonic antigen-related cell adhesion molecule 8; Anti-CD66b Antibody; Anti-CD67 Antibody; Anti-CGM6 Antibody; Anti-NCA-95 Antibody; anti-CEACAM8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 04
• Specificity: Human CEACAM8/CD66b
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.03%ProClin300
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CEACAM8 antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human CEACAM8/CD66b protein
• Conjugation: FITC
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CEACAM8/CD66b (rh CEACAM8/CD66b) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze!

Testing Data

(Profile of peripheral blood granulocytes analyzed by flow cytometry.)

Related Product Information for anti-CEACAM8 antibody

CEACAM8, also known as CD66b or NCA-95, is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic antigen family. There are four members in this family: CD66a, CD66b, CD66c, and CD66d. Members of CEACAM family are widely expressed especially on human neutrophils, and, depending on the tissue, capable of regulating diverse functions including tumor promotion, tumor suppression, angiogenesis, and neutrophil activation. Abnormal overexpression and downregulation of some CEACAMs have been described in tumor cells. Monoclonal antibodies grouped in the CD66 cluster recognize CEACAM members. Ectopic CD66 expression is commonly detected in B-cell lineage acute lymphoblastic leukemia (ALL). CEACAM8(CD66b) is also an activation marker for human granulocytes. However, its biological functions are largely unknown in eosinophils. It has been reported that CD66b is highly expressed on the surface of human peripheral blood eosinophils isolated from healthy individuals. Engagement of CD66b by mAb or a natural ligand, galectin-3, activated a Src kinase family molecule, hemopoietic cell kinase (Hck), and induced cellular adhesion, superoxide production, and degranulation of eosinophils. CD66b molecules were localized in lipid rafts, and disruption of lipid rafts or removal of the GPI anchor inhibited the adhesion and activation of eosinophils. Importantly, CD66b was constitutively and physically associated with a beta2 integrin, CD11b, and cross-linking of CD66b induced a striking clustering of CD11b molecules. Thus, CD66b molecules are involved in regulating adhesion and activation of eosinophils, possibly through their localization in lipid rafts and interaction with other cell surface molecules, such as CD11b. Binding of exogenous or endogenous carbohydrate ligands(s) to CD66b may be important in the release of proinflammatory mediators by human eosinophils.

References

Yoon J, et al. (2007) CD66b regulates adhesion and activation of human eosinophils. J Immunol. 179 (12): 8454-62.Skubitz KM, et al. (1996) CD6a, CD66b, CD66c, and CD66d each independently stimulate neutrophils. J Leukoc Biol. 60 (1): 106-17.Skubitz KM, et al. (2008) Inte rdependency of CEACAM-1, -3, -6, and -8 induced human neutrophil adhesion to endothelial cells. J Transl Med. 6: 78.Lasa A, et al. (2008) High expression of CEACAM6 and CEACAM8 mRNA in acute lymphoblastic leukemias. Ann Hematol. 87 (3): 205-11.

NCBI and Uniprot Product Information

• NCBI GI #: 21314600
• NCBI GeneID: 1088
• NCBI Accession #: NP_001807.2
• NCBI GenBank Nucleotide #: NM_001816.3
• UniProt Accession #: P31997; O60399; Q16574
• Molecular Weight: 38,154 Da
• NCBI Official Full Name: carcinoembryonic antigen-related cell adhesion molecule 8
• NCBI Official Synonym Full Names: carcinoembryonic antigen related cell adhesion molecule 8
• NCBI Official Symbol: CEACAM8
• NCBI Official Synonym Symbols: CD67; CGM6; CD66b; NCA-95
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 8
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 8
• Protein Family: Carcinoembryonic antigen-related cell adhesion molecule
• UniProt Gene Name: CEACAM8
• UniProt Synonym Gene Names: CGM6

Uniprot Description

Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:8776764, PubMed:2022629, PubMed:11590190). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:8776764, PubMed:2022629, PubMed:11590190). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764).