Rabbit anti-Human, Mouse JMJD6 Polyclonal Antibody | anti-Jmjd6 antibody

JMJD6, NT (JmjC Domain-containing Protein 6, Jumonji Domain-containing Protein 6, Protein PTDSR, Jmjd6, Ptdsr, Bifunctional Arginine Demethylase And Lysyl-Hydroxylase JMJD6, Histone Arginine Demethylase JMJD6, Peptide-lysine 5-dioxygenase JMJD6, Lysyl-hyd

Cat. Num. AAA628167

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: JMJD6; Polyclonal Antibody; NT (JmjC Domain-containing Protein 6; Jumonji Domain-containing Protein 6; Protein PTDSR; Jmjd6; Ptdsr; Bifunctional Arginine Demethylase And Lysyl-Hydroxylase JMJD6; Histone Arginine Demethylase JMJD6; Peptide-lysine 5-dioxygenase JMJD6; Lysyl-hyd; Anti -JMJD6; anti-Jmjd6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTDSR. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-Jmjd6 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:250-1:500
• Immunogen: Synthetic peptide selected from the N-terminal region of human PTDSR (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PTDSR (N-term) Pab in mouse heart, Hela, and HL60 cell line lysates. PTDSR (N-term)(arrow) was detected using the purified Pab.)

Related Product Information for anti-Jmjd6 antibody

Also known as JmjC domain-containing protein 6. This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. It functions in differentiation of multiple tissues during development, and in anti-inflammatory cytokine signaling. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have suggested that the protein may cross-react with a monoclonal antibody that recognizes the phosphatidylserine receptor and does not directly function in the clearance of apoptotic cells.

Product Categories/Family for anti-Jmjd6 antibody

Antibodies; Abs to Enzymes; Oxidase

NCBI and Uniprot Product Information

• NCBI GI #: 159572524
• UniProt Accession #: Q9ERI5; Q80TX1; A2AA26; A8Y5I2
• Molecular Weight: 46,567 Da
• NCBI Official Full Name: jumonji domain containing 6
• UniProt Protein Name: Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
• Protein Family: Bifunctional arginine demethylase and lysyl-hydroxylase
• UniProt Gene Name: Jmjd6
• UniProt Synonym Gene Names: Kiaa0585; Ptdsr
• UniProt Entry Name: JMJD6_MOUSE

Uniprot Description

JMJD6: Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses. Belongs to the JMJD6 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Apoptosis; Cell development/differentiation; EC 1.14.11.-; Oxidoreductase; Nucleolus

Cellular Component: nucleoplasm; nucleolus; plasma membrane; nucleus

Molecular Function: identical protein binding; histone demethylase activity (H4-R3 specific); RNA binding; metal ion binding; receptor activity; oxidoreductase activity; procollagen-proline dioxygenase activity; protein binding; single-stranded RNA binding; 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity; sulfonate dioxygenase activity; dioxygenase activity; iron ion binding; DNA-N1-methyladenine dioxygenase activity; histone demethylase activity (H3-R2 specific)

Biological Process: blood vessel development; erythrocyte development; transcription, DNA-dependent; multicellular organismal development; RNA splicing; heart development; T cell differentiation in the thymus; chromatin modification; recognition of apoptotic cell; peptidyl-lysine hydroxylation to 5-hydroxy-L-lysine; macrophage activation; regulation of nuclear mRNA splicing, via spliceosome; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; retina development in camera-type eye; sprouting angiogenesis; cell differentiation; kidney development; mRNA processing; apoptotic cell clearance; lung development