Mouse anti-Mouse CD59a Monoclonal Antibody | anti-CD59a antibody

CD59a, Mouse, mAb 7A6

Cat. Num. AAA584324

• Gene Names: Cd59a; Cd59; AA987121; protectin
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Synonyms: CD59a; Monoclonal Antibody; Mouse; mAb 7A6; Membrane attack complex inhibition factor; MACIF; MAC-inhibitory protein; Protectin; CD59; anti-CD59a antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 u/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 123

• Applicable Applications for anti-CD59a antibody: F, Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Application Use: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
• Immunogen: mCD59a-Fc
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-CD59a antibody

Product Description: The monoclonal antibody 7A6 (previously known as mCD59.3) recognizes mouse CD59a, a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 regulates the formation and function of the lytic C5b-9 complex by binding C8 and preventing the unfolding and membrane insertion of C9 and by binding C9 and restricting its polymerization.CD59 is a small (18 - 25 kDa) molecule, linked to the cell membrane through a glycosyl phosphatidylinositol (GPI) anchor and comprising 77 amino acids with a single N-linked carbohydrate group at Asn-18. Analogues of CD59 can be found in all species with similar structures and sizes. In contrast to all other species, mice have two genes encoding a CD59a and a CD59b protein. These two molecules are 63% identical at the amino acid level and share all major structural features. CD59a is broadly distributed on endothelia, erythrocytes, platelets and on numerous other cell types in organs, a distribution pattern resembling that of CD59 in other species. Expression of CD59b is restricted to germ cell elements in the testis and mature spermatozoa. Both CD59a and CD59b inhibit human and rodent complement with similar efficiency. CD59 may be involved in rheumatoid arthritis, motor nerve injury in the Guillain-Barr© sydrome and in other diseases where defective inhibition of complement activation on self tissue is involved. Furthermore, CD59 may play an important part in abrogating the effects of complement attack in renal disease. Its presence and protective effect have already been demonstrated on human renal cells.

References

1. Harris, C et al; Characterization of the mouse analogues of CD59 using novel monoclonal antibodies: tissue distribution and functional comparison. Immunology 2003, 109: 117
2. Williams, A et al; Deletion of the gene encoding CD59a in mice increases disease severity in a murine model of rheumatoid arthritis. Arthritis Rheum 2004, 50: 3035
3. Willison, H et al; The role of complement and complement regulators in mediating motor nerve terminal injury in murine models of Guillain -Barr© syndrome, J Neuroimmunol 2008, 201-202: 172
4. Yang, P et al; Expression and Modulation of RPE Cell Membrane Complement Regulatory Proteins. Invest Ophthalmol Vis Sci 2009, 50: 3473

NCBI and Uniprot Product Information

• NCBI GI #: 161484616
• NCBI GeneID: 12509
• NCBI Accession #: NP_001104530.1
• NCBI GenBank Nucleotide #: NM_001111060.2
• Molecular Weight: 13,648 Da
• NCBI Official Full Name: CD59A glycoprotein
• NCBI Official Synonym Full Names: CD59a antigen
• NCBI Official Symbol: Cd59a
• NCBI Official Synonym Symbols: Cd59; AA987121; protectin
• NCBI Protein Information: CD59A glycoprotein; MACIF; MAC-IP; CD59 antigen; MAC-inhibitory protein; membrane attack complex inhibition factor
• UniProt Protein Name: CD59A glycoprotein
• Protein Family: CD59A glycoprotein
• UniProt Gene Name: Cd59a
• UniProt Synonym Gene Names: Cd59; MAC-IP; MACIF
• UniProt Entry Name: CD59A_MOUSE

Uniprot Description

CD59A: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore.

Cellular Component: membrane; plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: complement binding

Biological Process: cell activation; negative regulation of angiogenesis; negative regulation of vascular endothelial growth factor receptor signaling pathway; negative regulation of complement activation; negative regulation of activation of membrane attack complex