CD59 / CD59A / MAC / IP recombinant protein

Recombinant Rat CD59 / CD59A / MAC / IP Protein (His tag)

Cat. Num. AAA2547365

• Gene Names: Cd59; Cd59a; Cd59b; MACIF; MACIP; MAC-IP
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CD59 / CD59A / MAC / IP; Recombinant Rat CD59 / CD59A / MAC / IP Protein (His tag); CD59; CD59 / CD59A / MAC / IP recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 126

• Application Notes: The recombinant rat CD59 /Fc comprises 89 amino acids and has a predicted molecular mass of 10.3 kDa. The apparent molecular mass of the protein is approximately 17 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Leu 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD59 / CD59A / MAC / IP recombinant protein

Background: CD59 glycoprotein, also known as 20 kDa homologous restriction factor, HRF20, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Description: A DNA sequence encoding the rat CD59 (P27274) (Met1-Asn100) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6978635
• NCBI GeneID: 25407
• NCBI Accession #: NP_037057.1
• NCBI GenBank Nucleotide #: NM_012925.1
• UniProt Accession #: P27274
• Molecular Weight: 13,790 Da
• NCBI Official Full Name: CD59 glycoprotein
• NCBI Official Synonym Full Names: CD59 molecule
• NCBI Official Symbol: Cd59
• NCBI Official Synonym Symbols: Cd59a; Cd59b; MACIF; MACIP; MAC-IP
• NCBI Protein Information: CD59 glycoprotein
• UniProt Protein Name: CD59 glycoprotein
• UniProt Gene Name: Cd59
• UniProt Synonym Gene Names: MAC-IP; MACIF

NCBI Description

may play a role in regulation of complement; may bind complement proteins C8 and C9 [RGD, Feb 2006]

Uniprot Description

CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3q32

Cellular Component: anchored to external side of plasma membrane; cell surface; compact myelin; extracellular space; focal adhesion; plasma membrane; sarcolemma; vesicle

Molecular Function: complement binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of activation of membrane attack complex; negative regulation of apoptosis; negative regulation of complement activation; negative regulation of cytolysis; positive regulation of T cell proliferation