Mouse CD54 Monoclonal Antibody | anti-CD54 antibody

anti-human CD54 (D2)-Biotin

Cat. Num. AAA666288

• Reactivity: Human, Primate
• Applications: Flow Cytometry, Functional Assay, ELISA
• Synonyms: CD54; Monoclonal Antibody; anti-human CD54 (D2)-Biotin; Monoclonal anti-human CD54(ICAM-1)/Biotin; anti-CD54 antibody

• Host: Mouse
• Reactivity: Human, Primate
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8.4A6
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-CD54 antibody: Flow Cytometry (FC/FACS), ELISA (EIA)
• Performance: Five x 105 cultured Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD54(8.4A6)/Biotin at a concentration of 5 ug/ml. Cells were then washed twice and incubated 45 minutes with 2 degree reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.66 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD54(8.4A6) antibody.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Human endothelial cells
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD54 antibody

Information: Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and monocytes. Antibody 8.4A6 recognizes the D2 domain of the CD54 molecule of 90 kd. Antibody 8.4A6 inhibits CD54 binding to LFA-1.

References

A.R. Berendt, et al, (1992) Cell 68: 71-81. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1548-1550. P.L. Reilly, et al, (1995) J Immunol 155: 529-532.