Mouse anti-Human CD11a Monoclonal Antibody | anti-CD11a antibody

anti-human CD11a-FITC

Cat. Num. AAA666294

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: CD11a; Monoclonal Antibody; anti-human CD11a-FITC; Monoclonal anti-human CD11a (LFA-1 integrin aL)/FITC; anti-CD11a antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 38
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.

• Applicable Applications for anti-CD11a antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: 1:50 (or use 1.6 ul of concentrated stock per 5 x 105-cell test)
• Performance: Five x 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ml of anti-CD11a/FITC at a 1:50 dilution (5 ug/ml). They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.79 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD11a antibody.
• Conjugation: FITC
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to 95% Immunoglobulin by SDS-PAGE, and reacted with FITC. Unconjugated FITC was removed from conjugate using a desalting column. Antibody conjugate is at 0.25 mg/ml with a Fluorescein/IgG molar ratio of 9.9.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Human monocytes
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD11a antibody

Information: Human CD11a (a L integrin) complexes with CD18 (b2 integrin) to form the LFA-1 heterodimer adhesion molecule which binds to three ICAM's 1, 2, and 3, also designated CD54, CD102, and CD50 respectively. LFA-1 is expressed on lymphocytes, monocytes and neutrophils and plays a role in cell-cell adhesion. Antibody 38 recognizes the I domain of the integrin alpha L subunit (CD11a) of about 180 kd. Antibody 38 blocks binding of ICAM-1 and ICAM-3 to LFA-1 at 5-10 mg/ml.

References

I. Dransfield & N. Hogg, (1989) EMBO J 12: 3759-3765. Leukocyte Typing V (S.F. Schlossman,et al, eds.) Oxford University Press, Oxford, (1995) p. 1581-1592. R.C. Landis, et al, (1994) J Cell Biol 126: 529-537. C.L. Holness, et al, (1995) J Biol Chem 270: 877-884.