Testing Data
Mouse anti-Human, Pig CD54 Monoclonal Antibody | anti-CD54 antibody
anti-human CD54 (D1)-Purified Preservative Free
Reactivity
Human, Pig
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Purified Preservative Free
Synonyms
CD54; Monoclonal Antibody; anti-human CD54 (D1)-Purified Preservative Free; Monoclonal anti-human CD54 (ICAM-1) Domain D1; anti-CD54 antibody
Host
Mouse
Reactivity
Human, Pig
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
15.2
Purity/Purification
Purified Preservative Free
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-CD54 antibody
Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA), FX
Performance
Five x 105 cultured Raji cells were incubated 45 minutes on ice with 80 ul of anti-CD54 antibody at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.22 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Human monocytes
Preparation and Storage
Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 6 months after shipping date when stored as recommended.
Product should retain activity for at least 6 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD54 antibody
Information: Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18
(LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and on monocytes. Antibody 15.2 recognizes the D1 domain of the CD54 molecule of 90 kd. Antibody 15.2 inhibits CD54 binding to LFA-1.
References
A.R. Berendt, et al, (1992) Cell 68: 71-81. I. Dransfield, et al, (1992) J Cell Biol 116: 1527-1535. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1548-1550. P.L. Reilly, et al, (1995) J Immunol 155: 529-532.
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Product Notes
The CD54 (Catalog #AAA666639) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD54 (D1)-Purified Preservative Free reacts with Human, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's CD54 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA), FX. Researchers should empirically determine the suitability of the CD54 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD54, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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