Mouse CD53 Monoclonal Antibody | anti-CD53 antibody

CD53 (TSPAN25) Mouse Monoclonal Antibody

Cat. Num. AAA439082

• Gene Names: CD53; MOX44; TSPAN25
• Reactivity: Human.; Does not react with Baboon and Horse.; Others not tested
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD53; Monoclonal Antibody; CD53 (TSPAN25) Mouse Monoclonal Antibody; CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25); TSPAN25; anti-CD53 antibody

• Host: Mouse
• Reactivity: Human.; Does not react with Baboon and Horse.; Others not tested
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: 161-2
• Specificity: Recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 219

• Applicable Applications for anti-CD53 antibody: Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Functional Studies (Order Ab without Azide); Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml)
• Cellular Localization: Cell Surface
• Immunogen: Stimulated human leukocytes
• Hu-Chromosome Location: 1p13
• Positive Control: Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Kishimoto T. et al., eds. Leukocyte Typing VI, p517-519 and p1153, Garland Publishing, Inc, New York and London, 1997. Maecker HT, et al. The tetraspanin superfamily: molecular facilitators. FASEB J 1997, 11:428-442. Rasmussen AM, et al. Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol 1994, 153:4997-5007. Szollosi J. et al. Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol 1996 Oct 1;157(7):2939-2946. Cao L. et al. Anti-CD53 monoclonal antibody induced LFA-1/ICAM-1-dependent and -independent lymphocyte homotypic cell aggregation Immunobiology 1997, 197:70-81

NCBI and Uniprot Product Information

• NCBI GI #: 10834972
• NCBI GeneID: 963
• NCBI Accession #: NP_000551.1
• NCBI GenBank Nucleotide #: NM_000560.3
• UniProt Accession #: P19397; Q5U0D6; B2R905
• Molecular Weight: 33-55kDa
• NCBI Official Full Name: leukocyte surface antigen CD53 isoform 1
• NCBI Official Synonym Full Names: CD53 molecule
• NCBI Official Symbol: CD53
• NCBI Official Synonym Symbols: MOX44; TSPAN25
• NCBI Protein Information: leukocyte surface antigen CD53
• UniProt Protein Name: Leukocyte surface antigen CD53
• Protein Family: Leukocyte surface antigen
• UniProt Gene Name: CD53
• UniProt Synonym Gene Names: MOX44; TSPAN25; Tspan-25
• UniProt Entry Name: CD53_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

CD53: May be involved in growth regulation in hematopoietic cells. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: immunological synapse; integral to plasma membrane; intercellular junction; plasma membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction