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Typical Testing Data

Rat Estrogen sulfotransferase, isoform 1 ELISA Kit | Sult1e1 elisa kit

Rat Estrogen sulfotransferase, isoform 1 ELISA Kit

Gene Names
Sult1e1; Ste; ST1E1; ESTSUL
Reactivity
Rat
Synonyms
Estrogen sulfotransferase; isoform 1; Rat Estrogen sulfotransferase; isoform 1 ELISA Kit; Sult1e1/EST-1/STE/SULT1E1/EC 2.8.2/EST-1/ESTMGC34459/estrone sulfotransferase/ST1E1/STE/STEestrogen sulfotransferase/Sulfotransferase 1E1/sulfotransferase family 1E; estrogen-preferring; member 1/Sulfotransferase; estrogen-preferringEC 2.8.2.4/SULT1E1; Sult1e1 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Sequence Length
295
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.312-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for Sult1e1 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Sult1e1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Sult1e1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Sult1e1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Sult1e1 can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,509 Da
NCBI Official Full Name
estrogen sulfotransferase
NCBI Official Synonym Full Names
sulfotransferase family 1E member 1
NCBI Official Symbol
Sult1e1
NCBI Official Synonym Symbols
Ste; ST1E1; ESTSUL
NCBI Protein Information
estrogen sulfotransferase
UniProt Protein Name
Estrogen sulfotransferase, isoform 1
Protein Family
UniProt Gene Name
Sult1e1
UniProt Synonym Gene Names
Ste; Ste1; EST-1; ST1E1
UniProt Entry Name
ST1E1_RAT

NCBI Description

isozyme that catalyzes the sulfation of estrogens, including beta-estradiol and estrone, but not of dehydroepiandrosterone, pregnenolone, cortisol or testosterone; plays a role in estrogen metabolism [RGD, Feb 2006]

Uniprot Description

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol ().

Research Articles on Sult1e1

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Product Notes

The Rat Sult1e1 sult1e1 (Catalog #AAA762386) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA762386 ELISA Kit recognizes Rat Sult1e1. It is sometimes possible for the material contained within the vial of "Estrogen sulfotransferase, isoform 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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