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Testing Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD52: Low Endotoxin (MBS215123))
Rat CD52 Monoclonal Antibody | anti-CD52 antibody
RAT ANTI HUMAN CD52
Gene Names
CD52; CDW52
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry
Synonyms
CD52; Monoclonal Antibody; RAT ANTI HUMAN CD52; anti-CD52 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
YTH34.5
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
61
Applicable Applications for anti-CD52 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunohistology Paraffin, Immunohistology Resin (RE)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Immunohistology - Frozen: Maximum Dilution: 1/20
Flow Cytometry: Maximum Dilution: 1/20
Immunohistology - Paraffin: Maximum Dilution: 1/20
Immunohistology - Frozen: Maximum Dilution: 1/20
Flow Cytometry: Maximum Dilution: 1/20
Immunohistology - Paraffin: Maximum Dilution: 1/20
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Histology Positive Control Tissue
Tonsil
Buffer Solution
Buffer Solution
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD52: Low Endotoxin (MBS215123))
Testing Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD52: Low Endotoxin (MBS215123))
Testing Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD52:RPE (MBS215125))
Testing Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD52:RPE (MBS215125))
Testing Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD52:FITC (MBS215124))
Testing Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD52:FITC (MBS215124))
Testing Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD52: Alexa Fluor 647 (MBS215122A647))
Testing Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD52: Alexa Fluor 647 (MBS215122A647))
Related Product Information for anti-CD52 antibody
Rat anti Human CD52 antibody, clone YTH34.5 reacts with the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small but heavily glycosylated peptide attached to the cell surface membrane via a GPI link (Xia et al. 1991). The apparent molecular mass of the native antigen on SDS-PAGE is 25-29kDa, considerably reduced following N-glycanase treatment (Rowan et al. 1998). CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies (Dearden et al. 2002; Pettitt et al. 2012).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
6,614 Da
NCBI Official Full Name
CAMPATH-1 antigen
NCBI Official Synonym Full Names
CD52 molecule
NCBI Official Symbol
CD52
NCBI Official Synonym Symbols
CDW52
NCBI Protein Information
CAMPATH-1 antigen; CD52 antigen (CAMPATH-1 antigen); CDW52 antigen (CAMPATH-1 antigen); HEL-S-171mP; cambridge pathology 1 antigen; epididymal secretory protein E5; epididymis secretory sperm binding protein Li 171mP; he5; human epididymis-specific protein 5
UniProt Protein Name
CAMPATH-1 antigen
Protein Family
UniProt Gene Name
CD52
UniProt Synonym Gene Names
CDW52; HE5; He5
UniProt Entry Name
CD52_HUMAN
Uniprot Description
CD52: May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
Protein type: Membrane protein, integral; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 1p36
Cellular Component: membrane; integral to plasma membrane
Biological Process: respiratory burst; elevation of cytosolic calcium ion concentration
Research Articles on CD52
Similar Products
Product Notes
The CD52 cd52 (Catalog #AAA215127) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD52 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunohistology Paraffin, Immunohistology Resin (RE). Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. Immunohistology - Frozen: Maximum Dilution: 1/20 Flow Cytometry: Maximum Dilution: 1/20 Immunohistology - Paraffin: Maximum Dilution: 1/20. Researchers should empirically determine the suitability of the CD52 cd52 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD52, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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