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Testing Data #1 (Staining of canine peripheral blood lymphocytes with Rat anti Canine MHC Class II: FITC (MBS214761))

Rat MHC CLASS II MONOMORPHIC Monoclonal Antibody

RAT ANTI DOG MHC CLASS II MONOMORPHIC

Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
MHC CLASS II MONOMORPHIC; Monoclonal Antibody; RAT ANTI DOG MHC CLASS II MONOMORPHIC; anti-MHC CLASS II MONOMORPHIC antibody
Ordering
For Research Use Only!
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
YKIX334.2
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-MHC CLASS II MONOMORPHIC antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Dog
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of canine peripheral blood lymphocytes with Rat anti Canine MHC Class II: FITC (MBS214761))

Testing Data #1 (Staining of canine peripheral blood lymphocytes with Rat anti Canine MHC Class II: FITC (MBS214761))
Related Product Information for anti-MHC CLASS II MONOMORPHIC antibody
Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2 recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Rat anti Dog MHC Class II immunoprecipitates an antigen of ~32/34kDa and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.

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Product Notes

The MHC CLASS II MONOMORPHIC (Catalog #AAA224446) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MHC CLASS II MONOMORPHIC can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the MHC CLASS II MONOMORPHIC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC CLASS II MONOMORPHIC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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