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Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD5:FITC)

Mouse CD5 Monoclonal Antibody | anti-CD5 antibody

MOUSE ANTI HUMAN CD5:FITC

Gene Names
CD5; T1; LEU1
Applications
Flow Cytometry, Functional Assay
Synonyms
CD5; Monoclonal Antibody; MOUSE ANTI HUMAN CD5:FITC; anti-CD5 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
MF7-14.5
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
495
Applicable Applications for anti-CD5 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C for one month or at -20 degree C for longer. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD5:FITC)

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD5:FITC)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD5:APC (MBS210011))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD5:APC (MBS210011))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD5:Biotin (MBS210326))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD5:Biotin (MBS210326))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD5:RPE (MBS213583))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD5:RPE (MBS213583))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD5 (MBS212107))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD5 (MBS212107))
Related Product Information for anti-CD5 antibody
Mouse anti Human CD5 antibody, clone MF7-14.5 recognizes the CD5 cell surface glycoprotein, a 67kDa antigen expressed by T lymphocytes and by a subset of B lymphocytes. CD5 is also expressed by tumor cells in cases of B-cell Chronic Lymphocytic Leukaemia (B-CLL)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
921
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,578 Da
NCBI Official Full Name
T-cell surface glycoprotein CD5
NCBI Official Synonym Full Names
CD5 molecule
NCBI Official Symbol
CD5
NCBI Official Synonym Symbols
T1; LEU1
NCBI Protein Information
T-cell surface glycoprotein CD5; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1
UniProt Protein Name
T-cell surface glycoprotein CD5
Protein Family
UniProt Gene Name
CD5
UniProt Synonym Gene Names
LEU1
UniProt Entry Name
CD5_HUMAN

Uniprot Description

CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cell proliferation; T cell costimulation; cell recognition

Research Articles on CD5

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Product Notes

The CD5 cd5 (Catalog #AAA210662) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD5 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the CD5 cd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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