Mouse anti-Human CD5 Monoclonal Antibody | anti-CD5 antibody

anti-human CD5-Biotin

Cat. Num. AAA666592

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD5; Monoclonal Antibody; anti-human CD5-Biotin; Monoclonal anti-human CD5/Biotin; anti-CD5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: UCHT2
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 463

• Applicable Applications for anti-CD5 antibody: Flow Cytometry (FC/FACS)
• Performance: Five x 105 ficoll prepared human peripheral blood mononuclear cells (PBMC) were washed and pre incubated 5 minutes with 300ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of product at 5 ug/ml. Cells were then washed twice and incubated 45 minutes with 20 ul of 2 degree reagent Streptavidin R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. A net 66% sub population of the cellsstained positive with a mean shift of 1.93 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Human thymocytes/Sezary T cells
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD5 antibody

Information: Human CD5 is believed to be an alternative signaling molecule found on all mature T cells, on most thymocytes and on a subpopulation of mature B cells expressing autoantibodies. The ligand for CD5 is CD72. Antibody UCHT2 recognizes epitope 2 of the CD5 molecule of approximately 67 kd. Antibody UCHT2 does not stimulate CD5+ PBMNC.

References

P.C.L. Beverly & R.E. Callard. "Protides of the Biological Fluids" (1981) Vol. 29: 653-658, H. Peeters (ed.), Pergamon Press, Oxford. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford (1989) p. 331-338. G.S. Wood & P.S. Freudenthal, (1992) Am J Pathol 141: 789-795. P.M. Lydyard, et al, (1993) Immunol Lett 38: 159-166.

NCBI and Uniprot Product Information

• NCBI GI #: 374720857
• Molecular Weight: 51,130 Da
• NCBI Official Full Name: CD5 molecule, partial
• UniProt Protein Name: CD5 molecule
• Protein Family: CD5 antigen
• UniProt Gene Name: CD5
• UniProt Entry Name: H6D9Y3_HUMAN

Uniprot Description

CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity

Biological Process: cell proliferation; receptor-mediated endocytosis; T cell costimulation; cell recognition