Testing Data
Mouse anti-Human CD56 Monoclonal Antibody | anti-CD56 antibody
anti-human CD56-Biotin
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, ELISA
Synonyms
CD56; Monoclonal Antibody; anti-human CD56-Biotin; Monoclonal anti-human CD56 (NCAM)/Biotin; anti-CD56 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
ERIC-1
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
848
Applicable Applications for anti-CD56 antibody
Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Performance
Five x 105 ficoll prepared human peripheral white blood cells were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block nonspecific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-CD56/Biotin at a concentration of 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed using by FACS. A net 15% sub population of the cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD56 antibody.
Conjugation
Biotin
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Human retinoblastoma tumor tissue, membrane fraction
Preparation and Storage
Store at 2 - 5 degree C. Freeze/thawing not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD56 antibody
Information: Human CD56 is an adhesion molecule from the Ig superfamily which is restricted to NK cells in the immune system. It is believed that NK cells form a first line of defense against tumor cells and cells infected with bacteria and viruses. Antibody ERIC-1 recognizes a stem region epitope of the CD56 molecule(4).
References
1) S.P. Bourne, et al, (1991) J Neuro-Oncol 10: 111-119. 2) T.L. Whiteside and R.B. Herberman (1994) Clinical & Diagnostic Laboratory Immunology. 3) H. Spits, et al, (1995) Blood 85: 2654-2670. 4) R. Gerardy-Schahn, M Eckhardt, (1994) Int J Cancer: Supp 8: 38-42.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
93,063 Da
NCBI Official Full Name
CD56
UniProt Protein Name
CD56
UniProt Gene Name
CD56
UniProt Entry Name
B7SAV9_TURTR
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Product Notes
The CD56 cd56 (Catalog #AAA666443) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD56-Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD56 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). Researchers should empirically determine the suitability of the CD56 cd56 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD56, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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