Testing Data
Mouse anti-Human CD8 Monoclonal Antibody | anti-CD8 antibody
anti-human CD8-Biotin
Gene Names
CD8A; CD8
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot, Immunocytochemistry
Synonyms
CD8; Monoclonal Antibody; anti-human CD8-Biotin; Monoclonal anti-human CD8/Biotin; anti-CD8 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
UCHT4
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
235
Applicable Applications for anti-CD8 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Performance
Five x 105 ficoll prepared peripheral blood mononuclear cells (PBMC) were washed and incubated 45 minutes on ice with 80 ul of anti-CD8/Biotin at 5 ug/ml. Cells were then washed twice and incubated 45 minutes with 2 degree reagent Streptavidin R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. A 25% sub population of the cells stained positive with a mean shift of 2.16 log10 fluorescent units when compared to a Mouse IgG2a/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated with 20 ul of 0.5 mg/ml unlabeled antibody.
Conjugation
Biotin
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Human thymocytes/Sezary T cells
Preparation and Storage
Store at 2 - 5 degree C. Freeze/thawing not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD8 antibody
Information: Human CD8 is a heterodimeric protein consisting of an alpha and beta chain, each about 33 kd. CD8 is expressed on most thymocytes and on about one third of peripheral blood T cells. CD8 is a co-receptor involved in antigen recognition and binds to the alpha2 and alpha3 domain of MHC Class I molecules.
References
A. Fischer, et al, (1983) Immunology 48: 177-186. R. Zamoyska, (1994) Immunity 1: 243-246. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 353-356. J. Sun, et al, (1995) J Exp Med 182: 1275-1280.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD8 antigen alpha polypeptide
NCBI Official Symbol
CD8A
NCBI Official Synonym Symbols
CD8
NCBI Protein Information
CD8 antigen alpha polypeptide; CD8 alpha chain; T-cell surface molecule; CD8 antigen, alpha polypeptide (p32)
Protein Family
Research Articles on CD8
Similar Products
Product Notes
The CD8 (Catalog #AAA666463) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD8-Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the CD8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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