Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD5 expression on human peripheral blood lymphocytes. Cells were stained with purified Rabbit anti-Human CD5, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.)
Rabbit anti-Human CD5 Monoclonal Antibody | anti-CD5 antibody
Recombinant Anti-CD5 Antibody, Rabbit Monoclonal
Gene Names
CD5; T1; LEU1
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD5; Monoclonal Antibody; Recombinant Anti-CD5 Antibody; Rabbit Monoclonal; CD5 Antibody; Rabbit MAb; CD5 molecule; Anti-LEU1 Antibody; Anti-T1 Antibody; anti-CD5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
039
Specificity
Human CD5
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD5 antibody
ELISA (EIA), Flow Cytometry (FC/FACS/FCM)
Application Notes
ELISA: 1:5000-1:10,000
FC/FACS/FCM: 1:25-1:100
FC/FACS/FCM: 1:25-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human CD5 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD5 (rh CD5).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD5 expression on human peripheral blood lymphocytes. Cells were stained with purified Rabbit anti-Human CD5, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD5 expression on human peripheral blood lymphocytes. Cells were stained with purified Rabbit anti-Human CD5, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.)
Related Product Information for anti-CD5 antibody
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.
Product Categories/Family for anti-CD5 antibody
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters. 134 (2): 104-12.Kirchgessner H, et al. (2001) The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 193 (11): 1269-84.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,578 Da
NCBI Official Full Name
T-cell surface glycoprotein CD5
NCBI Official Synonym Full Names
CD5 molecule
NCBI Official Symbol
CD5
NCBI Official Synonym Symbols
T1; LEU1
NCBI Protein Information
T-cell surface glycoprotein CD5; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1
UniProt Protein Name
T-cell surface glycoprotein CD5
Protein Family
UniProt Gene Name
CD5
UniProt Synonym Gene Names
LEU1
UniProt Entry Name
CD5_HUMAN
Uniprot Description
CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity
Biological Process: receptor-mediated endocytosis; cell proliferation; T cell costimulation; cell recognition
Research Articles on CD5
Similar Products
Product Notes
The CD5 cd5 (Catalog #AAA8104613) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD5 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS/FCM). ELISA: 1:5000-1:10,000 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the CD5 cd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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