Methionine Adenosyltransferase II Alpha (MAT2a) Recombinant Protein | MAT2a recombinant protein

Recombinant Methionine Adenosyltransferase II Alpha (MAT2a)

Cat. Num. AAA2018300

• Gene Names: MAT2A; MATA2; MATII; SAMS2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Methionine Adenosyltransferase II Alpha (MAT2a); Recombinant Methionine Adenosyltransferase II Alpha (MAT2a); MAT2a recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LRPDS KTQVTVQYMQ DRGAVLPIRV HTIVISVQHD EEVCLDEMRD ALKEKVIKAV VPAKYLDEDT IYHLQPSGRF VIGGPQGDAG LTGRKIIVDT YGGWGAHGGG AFSGKDYTKV DRSAAYAARW VAKSLVKGGL CRRVLVQVSY AIGVSHPLSI SIFHYGTSQK SERELLEIVK KNFDLRPGVI VRDLDLKKPI YQRTAAYGHF GRDSFPWEVP KKLKY
• Sequence Length: 395

• Applicable Applications for MAT2a recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Leu176~Tyr395 (Accession # P31153) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5174529
• NCBI GeneID: 4144
• NCBI Accession #: NP_005902.1
• NCBI GenBank Nucleotide #: NM_005911.5
• UniProt Accession #: P31153; Q53SP5; A8K511; D6W5L1
• Molecular Weight: 28.4kDa
• NCBI Official Full Name: S-adenosylmethionine synthase isoform type-2
• NCBI Official Synonym Full Names: methionine adenosyltransferase II, alpha
• NCBI Official Symbol: MAT2A
• NCBI Official Synonym Symbols: MATA2; MATII; SAMS2
• NCBI Protein Information: S-adenosylmethionine synthase isoform type-2; MAT 2; MAT-II; adoMet synthase 2; adoMet synthetase 2; methionine adenosyltransferase 2
• UniProt Protein Name: S-adenosylmethionine synthase isoform type-2
• Protein Family: S-adenosylmethionine synthase
• UniProt Gene Name: MAT2A
• UniProt Synonym Gene Names: AMS2; MATA2; AdoMet synthase 2; MAT 2; MAT-II
• UniProt Entry Name: METK2_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes. [provided by RefSeq, Jun 2011]

Uniprot Description

MAT2A: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. Belongs to the AdoMet synthase family.

Protein type: Amino Acid Metabolism - cysteine and methionine; Other Amino Acids Metabolism - selenoamino acid; Transferase; EC 2.5.1.6

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: methionine adenosyltransferase complex; cytosol

Molecular Function: protein binding; methionine adenosyltransferase activity; metal ion binding; ATP binding

Biological Process: methylation; xenobiotic metabolic process; S-adenosylmethionine biosynthetic process; one-carbon compound metabolic process