Mouse CD45RB Monoclonal Antibody

Anti-Hu CD45RB Alexa Fluor700

• Reactivity: Non-human primates, Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD45RB; Monoclonal Antibody; Anti-Hu CD45RB Alexa Fluor700; PTPRCR; T200RB; anti-CD45RB antibody

• Host: Mouse
• Reactivity: Non-human primates, Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: MEM-55
• Specificity: The antibody MEM-55 recognizes a siliadase-sensitive extracellular epitope of CD45RB, a 180-240kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD45RB antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: Alexa Fluor700
• Immunogen: Human thymocytes and T lymphocytes.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD45RB (MEM-55) Alexa Fluor 700.)

Related Product Information for anti-CD45RB antibody

CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.

References

*Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, Bemark M: Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011 Jul;90(1):5-19., URL: http://www.ncbi.nlm.nih.gov/pubmedterm=koethe%20s,*Horejsi V, Angelisova P, Bazil V, Kristofova H, Stoyanov S, Stefanova I, Hausner P, Vosecky M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34(1):23-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/2968928dopt=A,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL:,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL:,*Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987)., URL:
*Townsend KP, Vendrame M, Ehrhart J, Faza B, Zeng J, Town T, Tan J: CD45 isoform RB as a molecular target to oppose lipopolysaccharide-induced microglial activation in mice. Neurosci Lett. 2004 May 13;362(1):26-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/15147773ordin,*Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105(8):3295-302., URL: http://www.ncbi.nlm.nih.gov/pubmed/15626731ordina,*Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16(4):256-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/17107594ordina,*Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune Functional Application (FUNC)tion and disease. J Immunol. 2006 Mar 15;176(6):3417-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/16517710ordina,*Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44(15):3682-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/17524482ordina,*Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19, URL: http://www.ncbi.nlm.nih.gov/pubmed/18157742ordina