Goat TXNDC5 / ERP46 Polyclonal Antibody | anti-TXNDC5 antibody

Goat Polyclonal to Human TXNDC5 / ERP46

Cat. Num. AAA248798

• Gene Names: TXNDC5; ERP46; HCC-2; STRF8; PDIA15; UNQ364; ENDOPDI
• Reactivity: Gorilla, Human, Mouse; Predicted Reactivity: Gibbon, Monkey, Dog (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TXNDC5 / ERP46; Polyclonal Antibody; Goat Polyclonal to Human TXNDC5 / ERP46; Anti-TXNDC5 / ERP46 Antibody (Internal) IHC-plus; TXNDC5; ENDOPDI; ER protein 46; ERP46; PDIA15; STRF8; TLP46; UNQ364; HCC-2; Thioredoxin related protein; Thioredoxin-like protein p46; Human TXNDC5; anti-TXNDC5 antibody

• Host: Goat
• Reactivity: Gorilla, Human, Mouse; Predicted Reactivity: Gibbon, Monkey, Dog (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human TXNDC5. This antibody is expected to recognize both reported isoforms (NP_110437.2; NP_001139021.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 432

• Applicable Applications for anti-TXNDC5 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 50kD plus faint 35kD bands observed in Human Lymph Nodes lysates (calculated MW of 47.6kD according to NP_110437.2) and of 36.2kD according to NP_001139021.1. Recommended...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-RDLESLREYVESQLQ from an internal region of human TXNDC5 (NP_110437.2; NP_001139021.1). Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Elephant, Panda (100%); Gibbon, Monkey, Dog (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: TXNDC5 / ERP46 antibody was raised against synthetic peptide C-RDLESLREYVESQLQ from an internal region of human TXNDC5 (NP_110437.2; NP_001139021.1). Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Elephant, Panda (100%); Gibbon, Monkey, Dog (93%); Mouse, Rat, Hamster, Bat, Bovine, Rabbit, Horse (87%); Pig, Opossum (80%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TXNDC5 / ERP46 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(TXNDC5 antibody (0.1 ug/ml) staining of Human Lymph Nodes lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 42794771
• NCBI GeneID: 81567
• NCBI Accession #: NP_110437.2
• NCBI GenBank Nucleotide #: NM_030810.3
• UniProt Accession #: Q8NBS9; Q5TCQ0; Q8ND33; Q8TCT2; Q9BVH9; B2RDM2
• Molecular Weight: 36,178 Da
• NCBI Official Full Name: thioredoxin domain-containing protein 5 isoform 1
• NCBI Official Synonym Full Names: thioredoxin domain containing 5 (endoplasmic reticulum)
• NCBI Official Symbol: TXNDC5
• NCBI Official Synonym Symbols: ERP46; HCC-2; STRF8; PDIA15; UNQ364; ENDOPDI
• NCBI Protein Information: thioredoxin domain-containing protein 5; ER protein 46; thioredoxin related protein; thioredoxin-like protein p46; endoplasmic reticulum protein ERp46; endothelial protein disulphide isomerase; endoplasmic reticulum resident protein 46; protein disulfide isomerase family A, member 15
• UniProt Protein Name: Thioredoxin domain-containing protein 5
• Protein Family: Thioredoxin domain-containing protein
• UniProt Gene Name: TXNDC5
• UniProt Synonym Gene Names: TLP46; ER protein 46; ERp46
• UniProt Entry Name: TXND5_HUMAN

NCBI Description

This gene encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream MUTED (muted homolog) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

TXNDC5: Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide- isomerase deficiency in yeast. Belongs to the protein disulfide isomerase family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p24.3

Cellular Component: lysosomal lumen; endoplasmic reticulum lumen; endoplasmic reticulum

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: protein folding; cell redox homeostasis; post-Golgi vesicle-mediated transport; apoptotic cell clearance; negative regulation of apoptosis