Mouse anti-Human CD45RA Monoclonal Antibody

Anti-Hu CD45RA Pacific Blue

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD45RA; Monoclonal Antibody; Anti-Hu CD45RA Pacific Blue; PTPRCR; T200RA; anti-CD45RA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: MEM-56
• Specificity: The antibody MEM-56 reacts with an extracellular epitope of CD45RA, a 205-220kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.
• Purity/Purification: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Pacific Blue. Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD45RA antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Immunogen: Human thymocytes and T lymphocytes.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45RA (MEM-56) Pacific Blue™ antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human CD45RA positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45RA (MEM-56) Pacific Blue™ antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD45RA antibody

CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naïve T cells and normal plasma cells.

References

*Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha). 1989;35(5):289-97., URL: https://www.ncbi.nlm.nih.gov/pubmed/2534480dopt=A,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL:
*Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105(8):3295-302., URL: http://www.ncbi.nlm.nih.gov/pubmed/15626731ordina,*Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16(4):256-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/17107594ordina,*Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune Functional Application (FUNC)tion and disease. J Immunol. 2006 Mar 15;176(6):3417-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/16517710ordina,*Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44(15):3682-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/17524482ordina,*Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19, URL: http://www.ncbi.nlm.nih.gov/pubmed/18157742ordina