Rat CD45R Monoclonal Antibody | anti-CD45R antibody

RAT ANTI MOUSE CD45R:RPE

Cat. Num. AAA213565

• Gene Names: Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD45R; Monoclonal Antibody; RAT ANTI MOUSE CD45R:RPE; anti-CD45R antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: RA3-6B2
• Form/Format: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
• Sequence Length: 1291

• Applicable Applications for anti-CD45R antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR.; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 1 ml distilled water ; Perservative Stabilisers
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data

(Staining of mouse spleen cells with Rat anti Mouse CD45R:FITC (MBS210639))

Testing Data

(Staining of mouse spleen cells with Rat anti Mouse CD45R: Alexa Fluor 488 (MBS21398858A488))

Testing Data

(Staining of mouse spleen cells with Rat anti Mouse CD45R: Alexa Fluor 647 (MBS21398858A647))

Testing Data

(Staining of mouse spleen cells with Rat anti Mouse CD45R:RPE-Alexa Fluor 647 (MBS21398858P647))

Related Product Information for anti-CD45R antibody

Rat anti Mouse CD45R antibody, clone RA3-6B2 recognizes murine CD45R, a form of the CD45 antigen expressed by B cells and lytically active subsets of NK cells and non-MHC restricted CTL's. Rat anti Mouse CD45R antibody, clone RA3-6B2 immunoprecipitates the high molecular weight form of CD45 (220kD). Rat anti Mouse CD45R antibody, clone RA3-6B2 has been reported as being suitable for plp fixed paraffin embedded tissues (Whiteland et al.1995).

NCBI and Uniprot Product Information

• NCBI GI #: 341942176
• NCBI GeneID: 19264
• UniProt Accession #: P06800; Q61812; Q61813; Q61814; Q61815; Q78EF1; E9QLT5
• Molecular Weight: 130,622 Da
• NCBI Official Full Name: Receptor-type tyrosine-protein phosphatase C
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, C
• NCBI Official Symbol: Ptprc
• NCBI Official Synonym Symbols: loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C; leukocyte common antigen; lymphocyte antigen 5; lymphocyte common antigen
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: Ptprc
• UniProt Synonym Gene Names: Ly-5; L-CA; Ly-5
• UniProt Entry Name: PTPRC_MOUSE

Uniprot Description

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: EC 3.1.3.48; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine

Cellular Component: cell surface; focal adhesion; membrane; integral to plasma membrane; plasma membrane; integral to membrane; intracellular; cytosol; external side of plasma membrane

Molecular Function: heparan sulfate proteoglycan binding; heparin binding; protein binding; hydrolase activity; phosphoric monoester hydrolase activity; protein tyrosine phosphatase activity; phosphoprotein phosphatase activity; protein kinase binding

Biological Process: B cell proliferation; positive regulation of isotype switching to IgG isotypes; positive regulation of T cell mediated cytotoxicity; regulation of cell cycle; regulation of humoral immune response mediated by circulating immunoglobulin; activation of MAPK activity; protein amino acid dephosphorylation; T cell receptor signaling pathway; T cell proliferation; B cell receptor signaling pathway; leukocyte adhesion; positive regulation of MAPKKK cascade; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; response to gamma radiation; positive regulation of T cell mediated immunity; negative regulation of protein amino acid autophosphorylation; positive regulation of T cell proliferation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of gamma-delta T cell differentiation; defense response to virus; positive thymic T cell selection; T cell differentiation; regulation of B cell receptor signaling pathway; positive regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of peptidyl-tyrosine phosphorylation; substrate-bound cell migration, cell release from substrate; negative thymic T cell selection; positive regulation of antigen receptor-mediated signaling pathway; bone marrow development; heterotypic cell-cell adhesion; stem cell development; positive regulation of T cell differentiation; dephosphorylation; release of sequestered calcium ion into cytosol; B cell differentiation; regulation of B cell differentiation; negative regulation of protein kinase activity; positive regulation of alpha-beta T cell proliferation; hemopoietic progenitor cell differentiation; immunoglobulin biosynthetic process