Rat CD45 Monoclonal Antibody | anti-CD45 antibody

RAT ANTI MOUSE CD45

Cat. Num. AAA212005

• Gene Names: Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
• Synonyms: CD45; Monoclonal Antibody; RAT ANTI MOUSE CD45; anti-CD45 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: YW62.3
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1 mg/ml (varies by lot)
• Sequence Length: 1291

• Applicable Applications for anti-CD45 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD45 (Ly-5):Alexa Fluor 647 )

Testing Data

(Staining of mouse splenocytes with Rat anti Mouse CD45: FITC (MBS210598))

Testing Data

(Staining of mouse spleen with Rat anti Mouse CD45: RPE (MBS213540))

Testing Data

(Staining of mouse spleen with Rat anti Mouse CD45)

Testing Data

(Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5):RPE (MBS213539))

Testing Data

(Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5) (MBS212004))

Related Product Information for anti-CD45 antibody

Rat anti Mouse CD45 antibody, clone YW62.3 recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kDa glycoprotein expressed by all leucocytes. CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains (Komura et al. 1975), this was subsequently expanded to include a third allele encoding Ly5.3 (Shen et al. 1986). Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1 (Morse et al. 1987). Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb (Charbonneau et al. 1988; Zebedee et al. 1991). A number of different isoforms isoforms of CD45 are expressed on murine leucocytes depending on the pattern of altenative splicing of 3 exons termed A, B and C encoding regions of ~ 50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. (Birkeland et al. 1989). Rat anti mouse CD45 antibody, clone YW62.3 is reactive with all isoforms of murine CD45. N.B. Some reactivity with human tissue has been observed.

NCBI and Uniprot Product Information

• NCBI GI #: 341942176
• NCBI GeneID: 19264
• UniProt Accession #: P06800; Q61812; Q61813; Q61814; Q61815; Q78EF1; E9QLT5
• Molecular Weight: 130,622 Da
• NCBI Official Full Name: Receptor-type tyrosine-protein phosphatase C
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, C
• NCBI Official Symbol: Ptprc
• NCBI Official Synonym Symbols: loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C; leukocyte common antigen; lymphocyte antigen 5; lymphocyte common antigen
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: Ptprc
• UniProt Synonym Gene Names: Ly-5; L-CA; Ly-5
• UniProt Entry Name: PTPRC_MOUSE

Uniprot Description

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Membrane protein, integral; Receptor protein phosphatase, tyrosine

Cellular Component: focal adhesion; cell surface; membrane; integral to plasma membrane; integral to membrane; plasma membrane; intracellular; cytosol; external side of plasma membrane

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; protein binding; hydrolase activity; phosphoric monoester hydrolase activity; protein tyrosine phosphatase activity; phosphoprotein phosphatase activity; protein kinase binding

Biological Process: B cell proliferation; positive regulation of isotype switching to IgG isotypes; regulation of cell cycle; regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of T cell mediated cytotoxicity; activation of MAPK activity; protein amino acid dephosphorylation; T cell receptor signaling pathway; T cell proliferation; B cell receptor signaling pathway; leukocyte adhesion; positive regulation of MAPKKK cascade; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; response to gamma radiation; positive regulation of T cell mediated immunity; negative regulation of protein amino acid autophosphorylation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of T cell proliferation; positive regulation of gamma-delta T cell differentiation; defense response to virus; T cell differentiation; positive thymic T cell selection; regulation of B cell receptor signaling pathway; negative regulation of peptidyl-tyrosine phosphorylation; positive regulation of humoral immune response mediated by circulating immunoglobulin; substrate-bound cell migration, cell release from substrate; negative thymic T cell selection; positive regulation of antigen receptor-mediated signaling pathway; bone marrow development; heterotypic cell-cell adhesion; stem cell development; positive regulation of T cell differentiation; dephosphorylation; release of sequestered calcium ion into cytosol; B cell differentiation; regulation of B cell differentiation; negative regulation of protein kinase activity; positive regulation of alpha-beta T cell proliferation; hemopoietic progenitor cell differentiation; immunoglobulin biosynthetic process