Flow Cytometry (FC/FACS)
(
Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD45R (IVA103) Alexa Fluor 488 antibody (concentration in sample 3 ug/ml).)
Mouse anti-Cow CD45R Monoclonal Antibody | anti-CD45R antibody
Anti-Bov CD45R Alexa Fluor488
Reactivity
Cow
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry
Purity
The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD45R; Monoclonal Antibody; Anti-Bov CD45R Alexa Fluor488; PTPRCR; T200R; anti-CD45R antibody
Host
Mouse
Reactivity
Cow
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
IVA103
Specificity
The antibody IVA103 reacts with an extracellular epitope of CD45R antigen (bovine), a single chain type I transmembrane glycoprotein (member of the protein tyrosine phosphatase family), a restricted form of the CD45 family. CD45R is an essential regulator of T and B lymphocytes antigen receptor signaling and it is specifically expressed in hematopoietic cells.
Purity/Purification
The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Phosphate buffered saline (PBS) solution with 15mM sodium azide
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-CD45R antibody
Flow Cytometry (FC/FACS) (QC tested), Immunocytochemistry (ICC)
Application Notes
FC/FACS: Recommended dilution: 1-4ug/ml
Conjugation
Alexa Fluor488
Immunogen
Bovine leukocytes.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Flow Cytometry (FC/FACS)
( Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD45R (IVA103) Alexa Fluor 488 antibody (concentration in sample 3 ug/ml).)
Flow Cytometry (FC/FACS)
(
Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD45R (IVA103) Alexa Fluor 488 antibody (concentration in sample 3 ug/ml).)
Flow Cytometry (FC/FACS)
(Separation of bovine CD45R positive lymphocytes (red-filled) from CD45R negative cells (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD45R (IVA103) Alexa Fluor 488 antibody (concentration in sample 3 ug/ml).)
Flow Cytometry (FC/FACS)
(Separation of bovine CD45R positive lymphocytes (red-filled) from CD45R negative cells (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD45R (IVA103) Alexa Fluor 488 antibody (concentration in sample 3 ug/ml).)
Related Product Information for anti-CD45R antibody
CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.
References
*Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9267076dopt=A,*Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996)., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896204dopt=A,*Sopp P, Howard CJ: Investigation of PC36 (BoCD45R). Vet Immunol Immunopathol. 1996 Aug;52(4):271-4., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896215dopt=A
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Product Notes
The CD45R (Catalog #AAA1801969) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Bov CD45R Alexa Fluor488 reacts with Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CD45R can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (QC tested), Immunocytochemistry (ICC). FC/FACS: Recommended dilution: 1-4ug/ml. Researchers should empirically determine the suitability of the CD45R for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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