Human 5-Hydroxytryptamine Receptor 7 (HTR7) RTU ELISA Kit | HTR7 rtu elisa kit

Human 5-Hydroxytryptamine Receptor 7 (HTR7) ELISA Kit

Cat. Num. AAA4503807

• Gene Names: HTR7; 5-HT7
• Reactivity: Human
• Synonyms: 5-Hydroxytryptamine Receptor 7 (HTR7); Human 5-Hydroxytryptamine Receptor 7 (HTR7) ELISA Kit; 5-HT-X; Serotonin receptor 7; HTR7 rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HTR7. No significant cross-reactivity or interference between HTR7 and analogues was observed.
• Sequence Length: 445

• Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 34.2pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HTR7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HTR7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Stable for 12 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HTR7 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HTR7 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HTR7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HTR7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HTR7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HTR7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504547
• NCBI GeneID: 3363
• NCBI Accession #: NP_000863.1
• NCBI GenBank Nucleotide #: NM_000872.4
• UniProt Accession #: P34969; P78336; P78372; P78516; Q5VX01; Q5VX02; Q5VX03; B5BUP6
• Molecular Weight: 49,488 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 7 isoform a
• NCBI Official Synonym Full Names: 5-hydroxytryptamine receptor 7
• NCBI Official Symbol: HTR7
• NCBI Official Synonym Symbols: 5-HT7
• NCBI Protein Information: 5-hydroxytryptamine receptor 7
• UniProt Protein Name: 5-hydroxytryptamine receptor 7
• Protein Family: 5-hydroxytryptamine receptor
• UniProt Gene Name: HTR7
• UniProt Synonym Gene Names: 5-HT-7; 5-HT7

NCBI Description

The neurotransmitter, serotonin, is thought to play a role in various cognitive and behavioral functions. The serotonin receptor encoded by this gene belongs to the superfamily of G protein-coupled receptors and the gene is a candidate locus for involvement in autistic disorder and other neuropsychiatric disorders. Three splice variants have been identified which encode proteins that differ in the length of their carboxy terminal ends. [provided by RefSeq, Jul 2008]

Uniprot Description

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.