CDNF recombinant protein

CDNF Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121944

• Gene Names: CDNF; ARMETL1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CDNF; CDNF Protein; Human; Recombinant (His Tag); Human CDNF/ARMETL1 Protein (His Tag); ARMETL1 Protein; cerebral dopamine neurotrophic factor; CDNF recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu187

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Gln 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CDNF (NP_001025125.2)(Met1-Leu187) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CDNF recombinant protein

Background: Cerebral Dopamine Neurotrophic Factor (CDNF), also known as ARMETL1 (ARMET-like protein 1), is a secreted protein with eight conserved cysteine residues, predicting a unique protein fold and defining a new, evolutionarily conserved protein family. CDNF is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). CDNF/ARMETL1 is a evolutionary conserved protein which can protect and restore the function of dopaminergic neurons in the rat model of Parkinson's disease, suggesting that CDNF might be beneficial for the treatment of Parkinson's disease. CDNF is widely expressed in neurons in several brain regions including cerebral cortex, hippocampus, substantia nigra, striatum and cerebellum. Human CDNF is glycosylated and secreted from transiently transfected cells. CDNF promotes the survival, growth, and function of dopamine-specific neurons and is expressed in brain regions that undergo cocaine-induced neuroplasticity.

References

Choi JM, et al. (2011) Analysis of mutations and the association between polymorphisms in the cerebral dopamine neurotrophic factor (CDNF) gene and Parkinson disease. Neurosci Lett. 493(3): 97-101.Sun ZP, et al. (2011) Intracellular trafficking and secretion of cerebral dopamine neurotrophic factor in neurosecretory cells. J Neurochem. 117(1): 121-32.Lohoff FW, et al. (2009) Association analysis between polymorphisms in the conserved dopamine neurotrophic factor (CDNF) gene and cocaine dependence. Neurosci Lett. 453(3): 199-203.Lindholm P, et al. (2007) Novel neurotrophic factor CDNF protects and rescues midbrain dopamine neurons in vivo. Nature. 448(7149): 73-7.

NCBI and Uniprot Product Information

• NCBI GI #: 82880674
• NCBI GeneID: 441549
• NCBI Accession #: NP_001025125.2
• NCBI GenBank Nucleotide #: NM_001029954.2
• UniProt Accession #: Q49AH0; A2RUU0; B4DVW3
• Molecular Weight: 20,964 Da
• NCBI Official Full Name: cerebral dopamine neurotrophic factor
• NCBI Official Synonym Full Names: cerebral dopamine neurotrophic factor
• NCBI Official Symbol: CDNF
• NCBI Official Synonym Symbols: ARMETL1
• NCBI Protein Information: cerebral dopamine neurotrophic factor; ARMET-like protein 1; conserved dopamine neurotrophic factor; arginine-rich, mutated in early stage tumors-like 1
• UniProt Protein Name: Cerebral dopamine neurotrophic factor
• Protein Family: Cerebral dopamine neurotrophic factor
• UniProt Gene Name: CDNF
• UniProt Synonym Gene Names: ARMETL1
• UniProt Entry Name: CDNF_HUMAN

Uniprot Description

ARMETL1: Trophic factor for dopamine neurons. Prevents the 6- hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra. Belongs to the ARMET family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: extracellular region

Molecular Function: growth factor activity